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The transcriptional response of Arabidopsis thaliana cell suspensions following treatment with the stress hormone methyl jasmonate (MeJA) was monitored over time 16 hours after subcultivation. Three time points were included: 30 minutes, 2 hours and 6 hours after elicitation with 50µm MeJA or DMSO a...
ORGANISM(S): Arabidopsis thaliana 
A PSB-D wild type Arabidopsis thaliana cell culture (Arabidopsis Biological Resource Center stock: CCL84840) was grown after subculturing for one week and divided into separate 10 mL samples for 24 h. Samples were then treated with 50 µM RubNeddin1 (RN1, Chembridge ID 6389186), 2,4-D, or an equal vo...
ORGANISM(S): Arabidopsis thaliana 
A major role of NINJA is to repress root jasmonate signalling and allow normal cell elongation. To further characterize the transcriptional changes occurring in the root in the absence of a NINJA-dependent repression mechanism, we conducted a microarray analysis of ninja-1 versus WT roots. Root tran...
ORGANISM(S): Arabidopsis thaliana 
RNA was isolated from 14 day old Arabidopsis grxs17 seedlings in three biological repeats using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). A TruSeq RNA-Seq library (Illumina) was compiled and sequenced as 50-bp single read using Illumina HiSeq 2000. Control samples can be foun...
ORGANISM(S): Arabidopsis thaliana 
To identify the direct target genes of PPD2, tandem chromatin affinity purification (TChAP, Verkest et al. (2014)), a variant of chromatin immunoprecipitation (ChIP) in which tandem affinity tags are used instead of epitope tags, followed by sequencing (TChAP-Seq) was performed. An Arabidopsis cell ...
ORGANISM(S): Arabidopsis thaliana 
NINJA (At4g28910) and TOPLESS proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress- and growth-...
ORGANISM(S): Arabidopsis thaliana 
To understand how the NAC transcription factor KIL1 regulates age-induced senescence and cell death in maize silks, we need to get a genome-wide view on its downstream targets. We propose to compare the transcriptome profiles of GOF and LOF transgenic silk tissue with the profile of wild-type B104 s...
ORGANISM(S): Zea mays 
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