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TranscriptomicsTranscription profiling of rat PC12 cells treated with cholecystokinin for 2, 4, 8, and 16 hours
1 x 106 PC12-CCK-2R cells/100 mm or 1 x 106 PC12 cells/100 mm were seeded in DMEM containing 0.5% serum for 2 days before cells were untreated or treated with 200 nM CCK-8S for 2, 4, 8, and 16 hours. Total cellular RNA was isolated. Three independent series containing RNA from four 100 mm petri dis...
ORGANISM(S): Rattus norvegicus 
2007-11-09 | E-MEXP-1169 | biostudies-arrayexpress
TranscriptomicsMicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation
MicroRNAs (miRNAs) are small non-coding RNAs that control protein expression through translational inhibition or mRNA degradation. MiRNAs have been implicated in diverse biological processes such as development, proliferation, apoptosis and differentiation. Upon treatment with nerve growth factor (N...
ORGANISM(S): Rattus norvegicus 
2012-05-15 | E-GEOD-32122 | biostudies-arrayexpress
TranscriptomicsGlobal expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained MEK/ERK and AP-1 activation during PC12 differentiation.
Neuronal differentiation of PC12 cells in response to NGF is a prototypical model in which signal duration determines a biological response. Sustained ERK activity induced by NGF, as compared to transient activity induced by EGF, is critical to the differentiation of these cells. To characterize the...
ORGANISM(S): Rattus norvegicus 
2011-11-13 | E-GEOD-33639 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile in PC12 cells under NGF treatment and deprivation
miRNA profiling of PC12 rat pheochromocytoma cells during NGF-induced differentiation and apoptosis of differentiated cells after 24h of NGF deprivation. miRNA expression in differentiating and deprivated cells was compared to untreated and terminally differentiated PC12 cells respectivelly. Five-c...
ORGANISM(S): Rattus norvegicus 
2013-05-11 | E-GEOD-46827 | biostudies-arrayexpress
TranscriptomicsSerial analysis of chromatin occupancy (SACO) of forskolin-induced mRNA vs. an inactive control in the rat PC12 cell pheochromocytoma cell line to identify CREB targets
PC12 cells (passage 22) were cultured at 5% CO2 in a 37 C incubator. The growth medium was DMEM (high glucose, Invitrogen) supplemented with 25mM HEPES, 10% FCS, 5% FBS and 1x Pen/Strep (Invitrogen). 1x10^6 cells were starved overnight in DMEM +25 mM HEPES and treated with 10 uM 1,9 dideoxy forskoli...
ORGANISM(S): Rattus norvegicus 
2007-09-18 | E-GEOD-2071 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses
The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 ...
ORGANISM(S): Rattus norvegicus 
2009-04-29 | E-GEOD-5073 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat FGFR3-K650E mutant and wild type PC12 cells
To investigate signaling pathways activated by FGFR3 mutations through an analysis of altered expression of downstream transcriptional targets, we performed gene expression profiling of stably transfected inducible PC12 cells expressing a chimeric receptor (PFR3) consisting of the extracellular doma...
ORGANISM(S): Rattus norvegicus 
2007-10-08 | E-GEOD-2606 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat adrenal medulla and PC12 cell culture to investigate genetic networks regulated by short chain fatty acids
Genome-wide expression profiling showed significant overlap of the genetic networks regulated by butyrate in vivo (rat adrenal medulla) and in vitro (PC12 cells) including TH and other neurotransmitter-related genes (DBH, AADC, GTPCH, ppEnk, NPY). Experiment Overall Design: Studies in PC12 cells wer...
ORGANISM(S): Rattus norvegicus 
2009-11-14 | E-GEOD-15887 | biostudies-arrayexpress
TranscriptomicsEpigenomics of neural cells: REST-induced down- and up-regulation of gene expression in a two clone PC12 cell model.
Cell epigenomics depends on the marks released by transcription factors operating via the assembly of complexes that induce focal changes of DNA and histone structure. Among these factors is REST, a repressor that, via its strong decrease, governs both neuronal and neural cell differentiation and sp...
ORGANISM(S): Rattus norvegicus 
2015-08-31 | E-GEOD-59946 | biostudies-arrayexpress
TranscriptomicsNGF and proNGF activate functionally distinct mRNAs in PC12 cells: an early gene expression profiling
The biological activities of NGF and of its precursor proNGF are quite distinct, due to different receptor binding profiles, but nothing is known about how proNGF regulates gene expression. We performed experiments to address this question, by verifying whether a proNGF specific transcriptional sign...
ORGANISM(S): Rattus norvegicus 
2012-01-09 | E-GEOD-26528 | biostudies-arrayexpress
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