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ProteomicsGlobal proteomics proteasome mutants
Temperature sensitive(TS)missense mutants have been foundational in the characterization of the function of essential genes due to their pronounced cellular phenotypes that are likely associated with protein functional disruption. However, an unbiased approach for the analysis of the biochemical and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-03 | PXD017223 | Pride
Yeast Tmt Proteasome Tpp 
ProteomicsmTPP of temperature sensitive proteasome mutants
Temperature sensitive (TS) missense mutants have been foundational in the characterization essential gene function. However, an unbiased approach for the analysis of the biochemical and biophysical changes in TS missense mutants within the context of their functional proteomes is lacking. We applied...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-03 | PXD017222 | Pride
Tpp Yeast Proteasome Lc-ms/ms 
ProteomicsAffinity Purification Trigger with mTPP
We have applied a BASIL-like approach to increase the signal of low abundance peptides to the study of protein complexes. To accomplish this, we incorporated an affinity purified cleavage and polyadenylation factor (CPF) complex as a “trigger” into our mTPP workflow at a trigger sample to lowest hea...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD020689 | Pride
Ap-ms Tpp Lc-ms/ms 
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