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MetabolomicsMapping the biosynthetic pathway of a hybrid polyketide-nonribosomal peptide in a metazoan
Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NR...
2021-06-22 | MTBLS2313 | MetaboLights
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080274 | MassIVE
ProteomicsEfficient micro-scale basic reverse phase peptide fractionation for global and targeted proteomics
This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed prote...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-02-09 | MSV000079500 | MassIVE
ProteomicsDevelopment of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
Development of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-21 | MSV000087860 | MassIVE
ProteomicsTrimodal chromatography enables facile peptide fractionation
We evaluated the utility of a trimodal stationary phase (Trinity P1) in offline peptide fractionation, and found it performed similarly to ion exchange or high pH reversed phase based techniques. It is orthogonal to low pH reversed phase and uses MS compatible mobile phase solvents.
ORGANISM(S): Homo sapiens (Human) 
2017-08-07 | PXD006188 | Pride
Peptide fractionation Proteomics Trinity p1 Orthogonality 2d-lc Pancreatic cancer 
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo sapiens (Human) 
2015-07-08 | PXD002389 | Pride
Hek293 Ipg fractionation 
OtherIPG strip-based peptide fractionation for shotgun proteomics.
Data from ProteomeXchange, PXD ID: PXD002389. Experiment: Bibo_20130621_CHS_IEF_3-10linear_24slices, file: Bibo_20130621_CHS_IEF_3-10linear_24slices_20.mzml. Published as part of Methods Mol Biol. 2014;1156:67-77 . From the Abstract: {{i}} Efficient fractionation of peptides is an essential prerequ...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016255 | GPMDB
Resource Reanalysis 
ProteomicsCMMB-based isopropanol gradient peptide fractionation (CIF) enables rapid and robust off-line peptide mixture fractionation in bottom-up proteomics
HEK293 cells lysates fractionated by various different methods
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-05-22 | MSV000085458 | MassIVE
ProteomicsComparison of fractionation proteomics for local SWATH library building
For data-independent acquisition by means of sequential window acquisition of all theoretical fragment ion spectra (SWATH), a reference library of data-dependent acquisition (DDA) runs is typically used to correlate the quantitative data from the fragment ion spectra with peptide identifications. Th...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006190 | Pride
Library building Comparative study Swath-ms Fractionation proteomics Hesc 
ProteomicsIn-depth proteome analysis of human ascites using high-pH peptide fractionation
For in-depth profiling, ascites from patients with benign disease or gastric cancer were subjected to high-resolution mass spectromtery analysis. By high-pH peptide fractionation, 3481 protein groups were identified in 72 LC-MS/MS runs.
ORGANISM(S): Homo sapiens (Human) 
2018-04-16 | PXD003351 | Pride
Ascites High-ph peptide fractionation Gastric cancer 
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