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TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
TranscriptomicsRapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins
Background: Exosomes and extracellular vesicles (EVs) are increasingly recognized as important sources of biomarkers for disease study and diagnosis. Results: A synthetic peptide, Vn96, allows for capture of EVs from biological fluids using basic laboratory equipment. Conclusion: The Vn96-captured E...
ORGANISM(S): Homo sapiens 
2014-06-14 | E-GEOD-58464 | biostudies-arrayexpress
UnknownIgY-binding peptide screened from a random peptide library as a ligand for IgY purification.
Not available
S-EPMC5637892 | biostudies-literature
ProteomicsAn mRNA-based workflow for validation of Neo-Epitope Presentation by HLA-I/Peptide Affinity Purification
Human leukocyte antigen (HLA) class I restricted neo-epitopes play a key role in inducing an adaptive cytotoxic T lymphocyte response against cancer. To enhance therapeutic and personalized cancer vaccination strategies, the identification of these cancer-specific neo-epitopes is essential. Current ...
ORGANISM(S): Homo sapiens (Human) 
2025-06-23 | PXD062551 | Pride
P53 epitope Hla-a*0201 Immunopeptidomics Mrna 
ProteomicsTECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples
High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduc...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-01-25 | PXD018444 | Pride
Peptide purification Ppspe Cell culture Fedbatch cultivation High-throughput proteomics Sample preparation 
ProteomicsAffinity peptide ligands: New tools for chasing non-canonical N-phosphoproteome
Protein N-phosphorylation enrichment is faced with great difficulties due to the intrinsic instability of the N-P bond, which has seriously hindered its biological function unraveling. Current methods for N-phosphopeptides enrichment are challenging to fulfil high selectivity for all types of N-phos...
ORGANISM(S): Homo Sapiens Escherichia Coli Mus Musculus 
2024-12-12 | PXD058797 |
TranscriptomicsRapid, high efficiency isolation of pancreatic ß-cells
The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed an exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional pancreatic ß-cells. By targeting the glucagon-like peptide-1 receptor, ß-cells were isolate...
ORGANISM(S): Mus musculus 
2015-09-04 | E-GEOD-64508 | biostudies-arrayexpress
ProteomicsLL10000 hybridization
The screening of a previously reported fluorescein labelled 10,000 member PNA encoded peptide library allowed information on the interaction between the peptide-ligands and the cell surface receptors to be extracted, identified new peptide ligands for cell surface receptors, and gave crucial informa...
ORGANISM(S): Homo sapiens 
2011-11-08 | E-MEXP-3104 | biostudies-arrayexpress
TranscriptomicsAgo-APP: a GW peptide-based Argonaute affinity capture strategy
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of ...
ORGANISM(S): Arabidopsis thaliana 
2015-09-08 | E-GEOD-70553 | biostudies-arrayexpress
UnknownOverexpression and purification of human calcitonin gene-related peptide-receptor component protein in Escherichia coli.
Not available
S-EPMC1839922 | biostudies-literature
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