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ProteomicsComparing SILAC- and stable isotope dimethyl-labeling approaches for quantitative proteomics.
Comparison of SILAC and reductive dimethyl labeling; also applied both methods to affinity enrichment experiments with and without fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-29 | MSV000082868 | MassIVE
ProteomicsDiscovery of peptide biomarkers in male Eupolyphaga sinensis Walker (Tubiechong) by dimethyl labeling-based quantitative peptidomics analysis
Eupolyphaga sinensis Walker is a traditional Chinese medicinal insect. However, the peptide composition differences between male and female ES, which may underlie their distinct medicinal efficacies, remain largely unexplored. In this study, we performed a quantitative peptidomics analysis using sta...
ORGANISM(S): Eupolyphaga sinensis 
2026-09-07 | PXD080333 | Pride
Eupolyphaga sinensis peptide biomarkers dimethyl labeling Lc-ms/ms 
ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsEstablishment of dimethyl labeling-based quantitative acetylproteomics in Arabidopsis
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-03-14 | PXD006177 | Pride
Cscl density gradient (cdg) centrifugation Stable isotope dimethyl labelling Arabidopsis Squa-d Acetylproteomics 4c proteomic workflow Quantitative ptm proteomics 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2021-09-09 | PXD022794 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2023-05-10 | PXD022806 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
UnknownIn-depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immunoaffinity purification and stable isotope dimethyl labeling.
Not available
S-EPMC2808269 | biostudies-literature
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
ProteomicsAn Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of trypanosomes (RNAi knockdown data)
The AAA-ATPase Msp1 extracts mislocalized outer membrane proteins and thus contributes to mitochondrial proteostasis. Using pull down experiments, we show that trypanosomal Msp1 localizes to both the glycosome and the mitochondrial outer membrane, where it forms a stable complex with four outer memb...
ORGANISM(S): Trypanosoma brucei 
2023-07-26 | PXD039634 | Pride
Trypanosoma brucei Proteomics Peptide stable isotope dimethyl labeling Mitochondrial quality control Rnai-mediated protein knockdown 
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – IP input
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024079 | JPOST Repository
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