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Purpose: To compare RNASeq data of wild type and mutants under standard growth conditions (Stationary Phase)28C for 18 h. The mutants were found defective in genes encoding the following proteins: an alkaline metalloprotease secretion protein AprE, GidA, and a PIN domain protein. RNA-seq analysis...
ORGANISM(S): Serratia sp. SCBI 
Cell lysates were adjusted to 5% SDS, and 20 ugs was reduced with 10 mM DTT and alkylated with 25 mM iodoacetamide followed by digestion with 1:15 trypsin using an S-trap at 47 degC for 1 h. Lyophilized peptides were reconstituted at 0.5 ugs/uL, and a study pool QC sample was made by mixing equal vo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-12 | MSV000093088 | MassIVE
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