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TranscriptomicsTranscription profiling of E.coli BW25113 ymgB and W/T strains LBglu 24h biofilm cells
For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofil...
ORGANISM(S): Escherichia coli 
2008-06-15 | E-GEOD-6925 | biostudies-arrayexpress
ProteomicsQuantitative chemical proteomic profiling of phosphoprotein phosphatases
We have developed a chemical proteomic strategy for the systematic interrogation of endogenous phosphoprotein phosphatases (PPP) and their interacting proteins, including regulatory and scaffolding subunits, and substrates (the 'PPPome'). PPPs are captured using an immobilized non-selective PPP inhi...
ORGANISM(S): Neurospora Crassa (ncbitaxon:5141) Candida Albicans (ncbitaxon:5476) Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) Schizosaccharomyces Pombe (ncbitaxon:4896) 
2017-07-07 | MSV000081226 | MassIVE
ProteomicsGlobal phosphoproteomics for CRISPR/Cas9 mediated PTPN22 Ser325 mutagenesis on Jurkat cells
LQF of 3x FLAG PTPN22 WT Jurkat cells compared to S325A and S325E KI mutant Jurkat cells. WT or KI lines were stimulated with or without TCR engagement for 5 min.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-21 | MSV000093718 | MassIVE
ProteomicsImmunoprecipitated PTPN22 Phosphoproteomics from Jurkat Cells
3x FLAG tagged PTPN22 were immunoprecipitated from Jurkat cells stimulated by TCR for 5min, and the proteins were eluted by FLAG peptides. This study aims to explore the phosphorylation sites on PTPN22 phosphatase, identifying two phosphorylation sites Ser325 and Ser449.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-21 | MSV000093717 | MassIVE
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