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TranscriptomicsExpression analysis of Sphingomonas paucimobilis sp. EPA505 during biodegradation kinetics of various PAHs and detection of gene in a contaminated soil S3
Studying eight enzymes involved in the degradation of polycyclic aromatic hydrocarbons from the model strain Sphingomonas paucimobilis sp. EPA505 using a designed microarray of 8,048 probes. During the biodegradation kinetics with phenanthrene, fluoranthene or a mix of both pollutants, we identified...
ORGANISM(S): Sphingomonas paucimobilis 
2010-10-01 | E-GEOD-21402 | biostudies-arrayexpress
TranscriptomicsExperimental validation of HiSpOD probe design with complex background mRNA or 3 known antiense RNA and expression analysis on a contaminated groundwater sample
HiSpOD is a new efficient functional microarrays probe design algorithm especially dedicated for the microbial ecology and environmental studies. It was used to design 3392 probes targeting 21 genes involved in chlorinated solvent biodegradation pathways and synthesized on a nimblegen microarray. In...
ORGANISM(S): uncultured organism 
2011-04-13 | E-GEOD-21492 | biostudies-arrayexpress
TranscriptomicsMonitoring of functional gene responses to ERD (enhanced reductive dechlorination) from four TCE-contaminated sites
A functional microarray targeting 24 genes involved in chlorinated solvent biodegradation pathways has been developed and used to monitor the gene diversity present in four trichloroethylene (TCE) contaminated sites under ERD (enhanced reductive dechlorination) treatment. The microarray format provi...
ORGANISM(S): groundwater metagenome 
2012-04-02 | E-GEOD-28606 | biostudies-arrayexpress
TranscriptomicsMonitoring of functional gene responses to biostimulation from a TCE-contaminated site
A functional microarray targeting 24 genes involved in chlorinated solvent biodegradation pathways has been developed and used to monitor the gene expression in a contaminated site (site B) under ERD (enhanced reductive dechlorination) treatment. The microarray format provided by NimbleGen and used ...
ORGANISM(S): groundwater metagenome 
2012-04-02 | E-GEOD-28608 | biostudies-arrayexpress
TranscriptomicsCharacterization of the human host response to Encephalitozoon cuniculi infection
In order to study the molecular response of human foreskin fibroblast (HFF) cells to E. cuniculi parasite infection, kinetics microarray experiments targeting human genes expression have been performed during the parasite whole infection cycle. Infected HFF cells (IHFF; t=4h, 16h, 48h, 72h and 120h ...
ORGANISM(S): Homo sapiens 
2010-12-07 | E-GEOD-25880 | biostudies-arrayexpress
TranscriptomicsAssessing the importance of target type for oligonucleotide microarray experiments
In order to assess the effect on signal variability of the type of target used for oligonucleotide microarray experiments, we hybridized double and single stranded DNA of three genes on a Nimblegen High Density microarray. This slide carries specific probes (two lengths: 30 and 50 nucleotides, for a...
ORGANISM(S): Dehalococcoides sp. VS 
2010-07-27 | E-GEOD-23168 | biostudies-arrayexpress
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