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TranscriptomicsTimecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.
We have captured 100,000 single cells for single-cell RNAseq from whole mouse embryos during gastrulation and organogenesis, spanning days 6.5 to 8.5 of development, including embryonic and extraembryonic tissues. Cells were sampled every six hours, providing a continuous molecular characterisation ...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-6967 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-seq of mouse endothelial cells from three distinct embryonic locations
Endothelial cells are generated independently in the yolk sac, allantois and embryo proper. We dissected embryos from embryonic day 8.25 and captured individual cells from each of these positions within the embryo to investigate how their transcriptional signatures differ. Single-cell libraries were...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-6970 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development
Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells wer...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7324 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development
Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7325 | biostudies-arrayexpress
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