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TranscriptomicsTranscriptiion profiling by array of human ratiometric mixes of high quality RNA from different tissues using different labeling protocols to provide a mechanism for assessing process improvement
Ratiometric controls were prepared from commercial sources of high quality RNA from human tissues with distinctly different expression patterns.  The samples were processed using six different target labeling protocols and replicate datasets were generated on high density gene expression microarrays...
ORGANISM(S): Homo sapiens 
2011-04-08 | E-TABM-1091 | biostudies-arrayexpress
no omics definedTranscription profiling of fresh and frozen rat liver RNA samples to explore how RNA quality affects microarray results
To systemically explore how RNA quality affects microarray assay results, a set of rat liver RNA samples with a progressive change in RNA quality was generated either by thawing frozen tissue or by ex vivo incubation of fresh tissue.
ORGANISM(S): Rattus norvegicus 
2007-09-01 | E-MEXP-1069 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat mixed-tissue RNA samples using different labeling protocols
At one site (#10), three different batches of MTRRM (see E-TABM-16), were labeled with two different kits (Enzo and Affymetrix) and hybridized to two different Affymetrix Arrays (RAE230A and RAE230_2).
ORGANISM(S): Rattus norvegicus 
2008-04-16 | E-MEXP-1563 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat mixed-tissue RNA samples over 8.5 months to monitor processing drift
Process drift at one site was monitored by assaying the rat Mixed Tissue RNA Reference Material (see E-TABM-16) multiple times a week for 8.5 months.
ORGANISM(S): Rattus norvegicus 
2008-04-16 | E-MEXP-1565 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat mixed-tissue RNA samples in different laboratories to monitor proficiency
A pilot program for monitoring proficiency in microarray facilities using three replicates of two different RNA sources. Participating laboratories prepared and hybridized targets from the six RNA samples using their own protocols. The entire process was repeated three times over a nine-month peri...
ORGANISM(S): Rattus norvegicus 
2008-09-12 | E-MEXP-1568 | biostudies-arrayexpress
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