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TranscriptomicsIdentification of miRNAs and their target genes in developing seeds of soybean by deep sequencing
In our study, small RNA library and degradome library were constructed from developing soybean seeds for deep sequencing. We identified 26 new miRNAs in soybean by bioinformatic analysis, and further confirmed their expression by stem-loop RT-PCR. The miRNA star sequences of 38 known miRNAs and 8 ne...
ORGANISM(S): Glycine max 
2010-11-11 | E-GEOD-25260 | biostudies-arrayexpress
TranscriptomicsIdentifying conserved and novel miRNAs in developing seeds of Brassica napus using deep sequencing [mRNA-seq]
MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. The miRNA transcriptome of B. napus is currently poorly...
ORGANISM(S): Brassica napus 
2014-08-01 | E-GEOD-38018 | biostudies-arrayexpress
TranscriptomicsIdentifying conserved and novel miRNAs in developing seeds of Brassica napus using deep sequencing [small RNA-seq]
MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. The miRNA transcriptome of B. napus is currently poorly...
ORGANISM(S): Brassica napus 
2014-08-01 | E-GEOD-38019 | biostudies-arrayexpress
TranscriptomicsStress-Responsive MicroRNAs in Populus
MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become a subject of intense study. They are a class of post-transcriptional, negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory roles in tree’s responses to the...
ORGANISM(S): Populus trichocarpa 
2010-05-26 | E-GEOD-10582 | biostudies-arrayexpress
TranscriptomicsIdentification of miRNAs and their target genes in developing maize ears by deep sequencing
In plants, MicroRNAs (miRNAs) are a new class of endogenous small RNAs that play essential regulatory roles in plant growth, development and stress response. Extensive studies of miRNAs have been performed in model plants such as rice, Arabidopsis thaliana and other plants. However, the number of mi...
ORGANISM(S): Zea mays 
2014-10-22 | E-GEOD-47837 | biostudies-arrayexpress
TranscriptomicsDeep sequencing discovery of novel and conserved microRNAs in trifoliate orange (Citrus trifoliata)
MicroRNAs (miRNAs) play a critical role in post-transcriptional gene regulation. miRNAs have been shown to control many genes involved in various biological and metabolic processes. Deep sequencing technologies have facilitated identification of species-specific or lowly expressed as well as conser...
ORGANISM(S): Citrus trifoliata 
2010-08-17 | E-GEOD-22089 | biostudies-arrayexpress
TranscriptomicsA Comprehensive Genome-wide Study on Tissue- and Abiotic Stress-specific miRNAs in Triticum aestivum
Purpose: To identify abiotic stress responsive and tissue specific miRNAs at genome wide level in wheat (Triticum aestivum) Results: Small RNA libraries were constructed from four tissues (root, shoot, mature leaf and spikelets) and three stress treatments of wheat seedlings (control, high temperat...
ORGANISM(S): Triticum aestivum 
2014-08-20 | E-GEOD-53487 | biostudies-arrayexpress
TranscriptomicsApple miRNAs and tasiRNAs with novel regulatory networks
Background miRNAs and their regulatory functions have been extensively characterized in model species but whether apple has evolved similar or unique regulatory features remains unknown. Results We performed deep sRNA-seq and identified 23 conserved, 10 less-conserved and 42 apple-specific miRNAs o...
ORGANISM(S): Malus x domestica 
2012-05-29 | E-GEOD-36065 | biostudies-arrayexpress
TranscriptomicsIdentification and characterization of miRNAome in root, stem, leaf and tuber developmental stages of potato (Solanum tuberosum L.) by high-throughput sequencing
Purpose: MicroRNAs (miRNAs) are ubiquitous components of endogenous plant transcriptome. miRNAs are small, single-stranded and ~21 nt long RNAs which regulate gene expression at the post-transcriptional level and are known to play essential roles in various aspects of plant development and growth. P...
ORGANISM(S): Solanum tuberosum 
2014-01-09 | E-GEOD-52599 | biostudies-arrayexpress
TranscriptomicsIdentification and characterization of cold responsive microRNAs in tea plant (Camellia sinensis) based on high-throughput sequencing and their targets using degradome analysis [microarray]
Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the cold treatment of tea leaves. Subsequently, aligning these sequencing date with plant known miRNAs, we characterized 112 C. sinensis conserved miRNAs. In addition, 215 potential candidate mi...
ORGANISM(S): Camellia sinensis 
2015-05-14 | E-GEOD-53632 | biostudies-arrayexpress
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