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Mass spectrometric analysis of cesium chloride purified flagellins and pilins confirmed previously identified N-glycopeptides for FlgA1, revealed additional N-glycopeptides for FlgA1 and FlgA2 and characterized N-glycosylation of PilA1 and PilA2. Analyses were performed using different MS methods (w...
ORGANISM(S): Haloferax volcanii (Halobacterium volcanii) 
2020-05-11 | PXD011015 | Pride
To understand the impact of alternative translation initiation on a proteome, we performed the first study on protein turnover using positional proteomics and ribosome profiling to distinguish between N-terminal proteoforms of individual genes. Overall, we monitored the stability of 1,941 human N-te...
ORGANISM(S): Homo sapiens 
The free radical theory of ageing is based on the idea that reactive oxygen species (ROS) may lead to the accumulation of age-related protein oxidation. Since the majority of cellular ROS is generated at the respiratory electron transport chain, this study focuses on the mitochondrial proteome of th...
ORGANISM(S): Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina) 
2016-02-24 | PXD001023 | Pride
By combination of FACS sorting based on lipid sensitive Nile Red staining and small cell number proteomics the occurrence of population heterogeneity was analyzed. First population heterogeneity was identified by Nile Red staining and FACS in N. oceanica cells grown in nitrogen replete and nitrogen ...
ORGANISM(S): Nannochloropsis oceanica strain IMET1 
2019-02-15 | PXD008721 | Pride
Comparative transcriptome analyses revealed 69 genes exhibiting ?2-fold mRNA level changes in C. glutamicum DfkpA.Overall, 34 genes exhibited ?2-fold increased and 35 genes at least 0.5-fold decreased mRNA levels in JVO1 DfkpA. About half of these genes (32) encode for hypothetical/putative proteins...
ORGANISM(S): Corynebacterium glutamicum 
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