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Different attB or attP DNA libraries containing 7-bp random nucleotides were used for in vitro recombination mediated by the purified integrase from mv4 bacteriophage against their cognate wild-type attB or attP recombination site.
ORGANISM(S): Lactobacillus delbrueckii subsp. bulgaricus 
This study reports the first water-stressed transcriptome of Arundo donax L. (giant reed), a wild species that is emerging as one of the most promising biomass/bionergy species in mediterranean climates. Synchronized cohorts of giant reads cutting grown in hydroponic culture were subjected to water ...
ORGANISM(S): Arundo donax 
Although liganded nuclear receptors have been established to regulate RNA polymerase II (Pol II)-dependent transcription units, their role in regulating Pol III-transcribed DNA repeats remains largely unknown. Here we report that ~2-3% of the ~100,000-200,000 total human DR2 Alu repeats located in p...
ORGANISM(S): Homo sapiens 
The primary objective of this study was to evaluate response to a SIV DNA-based vaccine that was adminstered via vivo electroporation (EP) in rhesus macaques to further understand the molecular correlates of protection against SIV. In this study, rhesus macaques were immunized with a DNA vaccine inc...
ORGANISM(S): Macaca mulatta 
While liganded nuclear receptors are well established to regulate Pol II protein-coding transcription units, their role in regulation of DNA repeats remains largely unknown. Here, we report that ~2-3% of the ~1-200,000 human DR2 Alu repeats, particularly those in proximity to retinoic acid-activated...
ORGANISM(S): Homo sapiens 
Chromatin Immunoprecipitation of Centromeric Proteins, CenpA or CenpC, from human cell lines containing a neocentromere in band 13q21
ORGANISM(S): Homo sapiens 
We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity. Please see individual series. In short, for AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total ...
ORGANISM(S): Homo sapiens 
We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity. Please see individual series. For AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total RNA-Seq HE...
ORGANISM(S): Homo sapiens 
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