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ProteomicsFinding the optimal degassing condition for linear polyacrylamide coated capillaries used in capillary zone electrophoresis-mass spectrometry-based top-down proteomics
LPA-coated capillaries are widely used; however, the quality of the coating—which directly affects separation performance and analyte identification—can vary significantly depending on the individual performing the procedure. A critical factor in achieving consistent coating quality is the degassing...
ORGANISM(S): Homo sapiens (Human) 
2026-05-14 | PXD067278 | Pride
Degass Polyacrylamide-coated capillary Top-down proteomics Reproducibility Capillary zone electrophoresis 
ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
UnknownThermally-initiated free radical polymerization for reproducible production of stable linear polyacrylamide coated capillaries, and their application to proteomic analysis using capillary zone electrophoresis-mass spectrometry.
Not available
S-EPMC4690009 | biostudies-literature
ProteomicsSingle shot CZE-MS/MS for phosphoproteomics
The dependence of capillary zone electrophoresis separations on the charge state of analyte is useful for the analysis of many post-translational modifications in proteins. In this work, we coupled CZE to an Orbitrap Fusion Lumos Tribrid platform with advanced peak determination algorithm for phosph...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-13 | PXD012888 | Pride
Cze Phosphoproteomics Orbitrap fusion lumos tribrid Advanced peak determination algorithm 
UnknownAnalysis of Proteins, Protein Complexes, and Organellar Proteomes Using Sheathless Capillary Zone Electrophoresis - Native Mass Spectrometry.
Not available
S-EPMC5709234 | biostudies-literature
UnknownNeutral hydrophilic coatings for capillary electrophoresis prepared by controlled radical polymerization.
Not available
S-EPMC5131799 | biostudies-literature
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we ...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-07 | PXD042298 | Pride
Top-down proteomics  integral membrane protein  proteoform  capillary zone electrophoresis-mass spectrometry  mouse brain 
UnknownSurface-Confined Aqueous Reversible Addition-Fragmentation Chain Transfer (SCARAFT) Polymerization Method for Preparation of Coated Capillary Leads to over 10 000 Peptides Identified from 25 ng HeLa Digest by Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry.
Not available
S-EPMC5540315 | biostudies-literature
UnknownLarge-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry.
Not available
S-EPMC6235596 | biostudies-literature
UnknownPilot Evaluation of the Long-Term Reproducibility of Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of a Complex Proteome Sample.
Not available
S-EPMC11002928 | biostudies-literature
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