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MetabolomicsMapping the biosynthetic pathway of a hybrid polyketide-nonribosomal peptide in a metazoan
Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NR...
2021-06-22 | MTBLS2313 | MetaboLights
MetabolomicsPolyketide synthase-derived sphingolipids determine microbiota-mediated protection against pathogens in C. elegans
Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components of host-microbiota interactions, their exact role in microbiota-mediated protection is largely unexplored. We addressed this knowledge gap with the nematode ...
2025-05-06 | MTBLS8694 | MetaboLights
MetabolomicsPolyketide synthase-derived sphingolipids determine microbiota-mediated protection against pathogens in C. elegans

Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components of host-microbiota interactions, their exact role in microbiota-mediated protection is largely unexplored. We addressed this knowledge gap with the nemato...

2025-05-27 | MTBLS8440 | MetaboLights
ProteomicsMaximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies
Type I polyketide synthases (T1PKSs) hold an enormous potential as a rational production platform for the biosynthesis of speciality chemicals. However, despite the great progress in this field, the heterologous expression of PKSs remains a major challenge. One of the first measures to improve heter...
ORGANISM(S): Corynebacterium glutamicum Escherichia coli Pseudomonas putida KT2440 
2023-09-01 | PXD042749 | Pride
Codon optimization Heterologous expression Polyketide synthases 
ProteomicsUnnatural lactam production by reprogrammed modular polyketide synthases in Pseudomonas putida
In recent years, type I polyketide synthases (PKSs) has emerged as an enticing platform for the production of novel molecules by reconstituting their modules, expanding the chemical landscape beyond the limitations of conventional metabolic engineering. This study reports a retrobiosynthesis approac...
ORGANISM(S): Pseudomonas putida KT2440 
2025-01-22 | PXD045574 | Pride
Pseudomonas putida Unatural lactam Polyketide synthases 
ProteomicsEngineering polyketide stereocenters with ketoreductase domain exchanges
Polyketide synthases (PKSs) are versatile biosynthetic megasynthases capable of producing a diverse range of natural products with many applications, including in pharmaceuticals. The stereochemical precision of PKSs makes them a powerful tool for engineering tailored, unnatural polyketides; however...
ORGANISM(S): Escherichia coli 
2025-12-01 | PXD066826 | Pride
Polyketide synthases (pks) protein engineering  kr swaps jbei 
ProteomicsProteomics results from heterologous expression of Monocillin in Saccharomyces cerevisiae POP4
This experiment was carried out to obtain information about heterologous expression of the Monocillin and YNP1 polyketide synthases in Saccharomyces cerevisiae.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-05-13 | PXD076304 | Pride
Monocillin and ynp1 proteomics 
ProteomicsSolution study of a type I polyketide synthase from Mycobacterium tuberculosis: pks13, the condensase of mycolic acids
Background: Among the nineteen polyketide synthases encoded by the genome of Mycobacterium tuberculosis, Pks13 was identified as the condensase required for the final condensation step of two long acyl chains. This leads to mycolic acids, which are essential components of the cell envelope of Cory...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2022-05-11 | PXD029754 | Pride
Polyketide synthases Mycolic acids Bottom up Native ms Mycobacterium tuberculosis Pks13 
GenomicsThe PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis [ChIP-Seq]
The PhoPR two-component system is essential for virulence in Mycobacterium tuberculosis where it controls expression of approximately 2% of the genes, including those for the ESX-1 secretion apparatus, a major virulence determinant. Mutations in phoP lead to compromised production of pathogen-specif...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2014-06-02 | E-GEOD-54239 | biostudies-arrayexpress
TranscriptomicsThe PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis [RNA-Seq]
The PhoPR two-component system is essential for virulence in Mycobacterium tuberculosis where it controls expression of approximately 2% of the genes, including those for the ESX-1 secretion apparatus, a major virulence determinant. Mutations in phoP lead to compromised production of pathogen-specif...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2014-06-02 | E-GEOD-54240 | biostudies-arrayexpress
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