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MetabolomicsCharacterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation

Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged ...

2026-08-24 | MTBLS12801 | MetaboLights
ProteomicsImproving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis
This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of...
ORGANISM(S): Homo sapiens (Human) 
2018-08-31 | PXD010315 | Pride
Calcium phosphate fractionation  human milk whey proteins  human milk caseins  capillary electrophoresis  fraction collection 
ProteomicsProtein precipitation of diluted samples with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach
Proteomic approaches are extremely valuable in many fields of research, where mass spectrometry methods have gained an increasing interest especially due to the ability to perform quantitative analysis. Nonetheless, sample preparation prior to mass spectrometry analysis is of the utmost importance. ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-25 | PXD003653 | Pride
Protein precipitation  acetone  tca/acetone  swath 
MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
ProteomicsIn-depth investigation of low-abundant proteins in matured and filling stages seeds of Glycine max by a combination of protamine sulfate precipitation and TMT-based quantitative proteomic analysis
The presence of seed storage proteins is a major bottleneck in soybean seed proteome analysis despite the significant technical advancements in mass spectrometry and data analysis software. Our previous studies have shown that effective ways to improve the detection of low-abundance proteins in soyb...
ORGANISM(S): Glycine max 
2020-06-28 | PXD019155 | Pride
Low-abundant proteins Perseus Tandem mass tags Basic ph reversed phase Maxquant Filter-aided sample preparation Protamine sulfate precipitation Glycine max 
Genomicsgravesoil fungi under precipitation
fungi
PRJEB55784 | ENA
TranscriptomicsAgo2 associated gene transcripts in two Hodgkin lymphoma cell lines
The aim of the study is to identify miRNA targets in Hodgkin lymphoma cell lines. By immunoprecipitation of wild type Ago2, it is expected to pull down the Ago2 associated gene transcripts. Through microarray analysis, the Ago2 associated gene transcripts identified are expected to be miRNA targets....
ORGANISM(S): Homo sapiens 
2010-04-08 | E-GEOD-14409 | biostudies-arrayexpress
ProteomicsCanagliflozin regulates ferroptosis to ameliorate heart failure in rats with preserved ejection fraction
Chronic heart failure (CHF), with whether reduced ejection fraction (HFrEF) or preserved ejection fraction (HFpEF), is the end-stage of various cardiovascular diseases and severely affects the patients’ lifespan. There has been no effective drugs to improve clinical outcomes for a long time. Sine th...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-05-20 | PXD029031 | Pride
Sodium-glucose cotransporter 2 inhibitor Canagliflozin Heart failure Ferroptosis Lipid peroxidation 
UnknownForests buffer against variations in precipitation.
Not available
S-EPMC8457185 | biostudies-literature
TranscriptomicsTop1 and Top2-mediated replication fork integrity
DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chroma...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-05 | E-GEOD-8305 | biostudies-arrayexpress
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