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ProteomicsA semi-automated workflow for DIA-based global discovery to pathway-driven PRM analysis
Initial discovery-based analysis using data independent acquisition (DIA) can obtain deep proteome coverage with high data completeness; however, the development of targeted PRM assays based on subsequent bioinformatic predictions can be tedious and time-consuming because of the complexity of the ou...
ORGANISM(S): Homo sapiens (Human) 
2024-09-06 | PXD049405 | Pride
Dia Timstof Script Bruker Sem-automated Prm 
ProteomicsOn-board Preconcentration Microchip Capillary Electrophoresis Based TMT-PRM-LIVE for High-throughput Selectivity Profiling of Deubiquitinase Inhibitors
On-board Preconcentration Microchip Capillary Electrophoresis Based TMT-PRM-LIVE for High-throughput Selectivity Profiling of Deubiquitinase Inhibitors
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-03-24 | MSV000089122 | MassIVE
GenomicsProvidencia alcalifaciens strain:PRM-2
Providencia alcalifaciens strain:PRM-2 Genome sequencing and assembly
PRJNA437544 | ENA
ProteomicsWideband PRM: Highly Accurate and Sensitive Method for High-Throughput Targeted Proteomics
Targeted mass spectrometry (MS) approaches, which are powerful methods for uniquely and confidently quantifying a specific panel of proteins in complex biological samples, play a crucial role in validation and clinical translation of protein biomarkers discovered by global proteomic profiling. Commo...
ORGANISM(S): Homo sapiens (Human) 
2024-08-14 | PXD048271 | Pride
Prm Targeted proteomics Parallel reaction monitoring Absolute quantification 
ProteomicsPRM-MS quantitative analysis of isomeric N-glycopeptides derived from human serum haptoglobin of patients with cirrhosis and hepatocellular carcinoma
Comprehensive LC-PRM-MS approach where a targeted parallel reaction monitoring (PRM) strategy was coupled to a powerful LC system to study the microheterogeneity of serum haptoglobin (Hp) extracted from 15 patients with cirrhosis and 15 with hepatocellular carcinoma (HCC).
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023719 | Pride
N-glycopeptides Prm Hepatocellular carcinoma (hcc) Haptoglobin Cirrhosis 
ProteomicsIdentification and relative quantification of hFSH glycoforms in women sera by PRM MS based approach.
This study presents an alternative preliminary strategy to identify and quantify serum hFSH glycoforms based on immunopurification assay and mass spectrometry (MS) using parallel reaction monitoring (PRM) analysis. In this study, we provide MS-PRM data acquisition method for hFSH glycopeptides ident...
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD025585 | Pride
Prm-mass spectrometry  fsh  glycosylation 
MetabolomicsLiquid Chromatography-Mass Spectrometry Quantification of Phytochemicals in Withania somnifera Using Data-Dependent Acquisition, Multiple-Reaction-Monitoring, and Parallel-Reaction-Monitoring with an Inclusion List

Characterization of botanical extracts by mass spectrometry-based metabolomics analysis helps in determining the phytochemical composition that underlies their bioactivity and potential health benefits, while also supporting reproducibility of effects in clinical trials. The quantification of sev...

2024-07-10 | MTBLS10418 | MetaboLights
ProteomicsPRM of Ganoderma lucidum dikaryon and monokaryons
PRM was performed to validate TMT-based quantification of Ganoderma lucidum dikaryon and monokaryons proteome to probe the profiles of different biological processes and their coordination that contribute to the dikaryon growth advantage.
ORGANISM(S): Ganoderma lucidum 
2025-07-09 | PXD051874 | Pride
Monokaryon Ganoderma lucidum Prm Growth advantage Dikaryon 
ProteomicsLong-read Proteogenomics Internal Standard triggered PRM (LRP-IS-PRM)
Long-read proteogenomic data was used to create sample-matched protein database for WTC11 sample. This includes many potential alternative protein isoforms (major and minor isoforms) per gene. The IS-PRM method called Tomahto was tested versus DDA to demonstrate improved coverage of isoform-specific...
ORGANISM(S): Homo sapiens (Human) 
2024-07-17 | PXD050909 | Pride
Proteogenomics Is-prm Tomahto 
ProteomicsLong-read Proteogenomics Internal Standard triggered PRM (LRP-IS-PRM)
Long-read proteogenomic data was used to create sample-matched protein database for WTC11 sample. This includes many potential alternative protein isoforms (major and minor isoforms) per gene. The IS-PRM method called Tomahto was tested versus DDA to demonstrate improved coverage of isoform-specific...
ORGANISM(S): Homo sapiens (Human) 
2024-07-17 | PXD050904 | Pride
Proteogenomics Is-prm Tomahto 
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