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MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
MetabolomicsNPC1 regulates the distribution of phosphatidylinositol 4 kinases at Golgi and Lysosome membranes
Cholesterol and phosphoinositides (PI) are two critically important lipids that are found in cellular membranes and dysregulated in many disorders. Therefore, uncovering molecular pathways connecting these essential lipids may offer new therapeutic insights. We report that loss of function of lysoso...
2021-09-15 | MTBLS2612 | MetaboLights
MetabolomicsDNA nanomachines reveal an adaptive energy mode in confinement-induced amoeboid migration powered by polarized mitochondrial distribution

Energy metabolism is highly interdependent with adaptive cell migration in vivo. Mechanical confinement is a critical physical cue that induces switchable migration modes of the mesenchymal-to-amoeboid transition (MAT). However, the energy states in distinct migration modes, especially a...

2024-03-25 | MTBLS9657 | MetaboLights
MetabolomicsAlcohol-Induced Bone Loss: Driven by Dysregulated Spatial Distribution of Gut Microbiota and PGD2-IL17 Pathway-Mediated Osteoclast Activation

Purpose: Alcohol-induced damage to bone microstructure leads to alcoholic osteopenia (AOP). While prior studies have demonstrated alcohol's negative impact on bone density, the mechanisms by which alcohol induces osteoporosis through immune pathways, gut microbiota dysbiosis, and metabolic altera...

2025-03-28 | MTBLS11887 | MetaboLights
MetabolomicsPyruvate metabolism enzyme Dlat induces mitochondria protein hyperacetylation to limit fatty acid oxidation in the HFpEF heart

Increased protein acetylation is frequently observed in the failing heart, including in hearts with heart failure with preserved ejection fraction (HFpEF). However, the role of protein acetylation in cardiac metabolic impairments during the pathogenesis of HFpEF remains insufficiently investigate...

2025-09-01 | MTBLS12919 | MetaboLights
MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
MetabolomicsOGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesis

Acute Myeloid Leukemia (AML) commonly relapses after initial chemotherapy response. We assessed metabolic adaptations in chemoresistant cells in vivo before overt relapse, identifying altered branched-chain amino acid (BCAA) levels in patient-derived xenografts (PDX) and immunophenotypically iden...

2025-07-19 | MTBLS10255 | MetaboLights
MetabolomicsA multi-omics study on the metabolic reprogramming of a chlorophyll-deficient, high-protein Auxenochlorella pyrenoidosa mutant (CX41) generated by atmospheric and room temperature plasma (ARTP) mutagenesis.

This project aims to elucidate the molecular mechanism underlying the stable golden-yellow phenotype and enhanced protein accumulation in an Auxenochlorella pyrenoidosa mutant (CX41) generated by ARTP mutagenesis. Through comparative analysis between the wild-type (FACHB-9) and mutant s...

2025-12-26 | MTBLS13574 | MetaboLights
TranscriptomicsRec8 guides canonical Spo11 distribution along yeast meiotic chromosomes
Spo11-mediated DNA double strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled. The meiotic cohesin Rec8 has been implicated in regulating DSB formation, but little is known about the features of their interplay. To shed light on this point, we investigate...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-18 | E-GEOD-8422 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells
Recent studies have demonstrated that the Ten-eleven translocation (Tet) family proteins can enzymatically convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). While 5mC has been studied extensively, little is known about the distribution and function of 5hmC. Here we present a genome...
ORGANISM(S): Mus musculus 
2011-03-30 | E-GEOD-27613 | biostudies-arrayexpress
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