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MetabolomicsMetabolomic analysis of riboswitch containing E. coli recombinant expression system
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
MetabolomicsRibosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis

Translation fidelity is the limiting factor in the accuracy of gene expression. With an estimated frequency of 10-4, errors in mRNA decoding occur in a mostly stochastic manner. Little is known about the response of higher eukaryotes to chronic loss of ribosomal accuracy as per an increase in the...

2019-09-17 | MTBLS106 | MetaboLights
MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
MetabolomicsLarge neutral amino acid levels tune perinatal neuronal excitability and survival
Surprisingly little is known about the critical metabolic changes that neural cells have to undergo during development and how even mild, temporary shifts in this program can influence brain circuitries and behavior. Inspired by the discovery that mutations in SLC7A5, a transporter of metabolically-...
2023-03-28 | MTBLS6578 | MetaboLights
MetabolomicsMolecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics

Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Ba...

2021-10-29 | MTBLS1803 | MetaboLights
MetabolomicsThe metabolic background is a global player in Saccharomyces gene expression epistasis
The regulation of gene expression in response to nutrient availability is fundamental to the genotype-phenotype relationship. The metabolic-genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of gene expression. Here, we address the importance of the metaboli...
2016-12-16 | MTBLS168 | MetaboLights
MetabolomicsRare genetic variants affecting urine metabolite levels link population variation to inborn errors of metabolism

We investigated the cumulative contribution of rare, exonic genetic variants on the concentration of 1,487 metabolites and 53,714 metabolite ratios in urine by performing gene-based tests based on 226,233 variants from up to 4,864 participants of the German Chronic Kidney Disease (GCKD)...

2021-03-09 | MTBLS284 | MetaboLights
MetabolomicsElevated temperature accelerates leaf senescence and promotes leaf nitrogen transport to improve rice grain protein synthesis
Elevated temperatures during grain filling severely constrain rice yield and quality. Although additional nitrogen can mitigate the adverse effects of elevated temperature, both factors increase grain protein content, and the underlying physiological mechanisms remain poorly understood. Here, we con...
2026-06-17 | MTBLS14777 | MetaboLights
Metabolomics1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats

BACKGROUND: Protein undernutrition is prevalent mainly among elderly people, and it could develop diseases such as sarcopenia. Urinary metabolites may become biomarkers reflecting protein undernutrition, but this has been insufficiently investigated.

OBJECTIVE:

2021-05-27 | MTBLS1452 | MetaboLights
MetabolomicsCycloleucine disturbs functions of porcine Sertoli cells via modulating gene expression and metabolism

Cycloleucine (CL) is a methyl donor inhibitor. Our previous findings showed that CL could affect meiotic maturation and developmental potency of porcine oocytes by reducing nucleic acid N6-methyladenosine (m6A) epigenetic modification level. However, the effects of CL on porcine male reproduction...

2025-09-13 | MTBLS12990 | MetaboLights
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