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UnknownImproving the Depth and Reliability of Glycopeptide Identification Using Protein Prospector.
Not available
S-EPMC11851224 | biostudies-literature
ProteomicsAutoantibodies are abundant in all human sera: a demographic characterization via protein microarrays
Human serum samples were probed onto human protein microarrays in order to ascertain the number and diversity of naturally ocurring self-reactive IgG autoantibodies. In the study presented here, 166 human serum samples were probed onto human protein microarrays in order to identify self-reactive IgG...
ORGANISM(S): Homo sapiens 
2013-04-25 | E-GEOD-39087 | biostudies-arrayexpress
Unknown15N Metabolic Labeling Quantification Workflow in Arabidopsis Using Protein Prospector.
Not available
S-EPMC8885517 | biostudies-literature
ProteomicsIdentification of Novel Auto-antibodies in Type 1 Diabetic Patients using a High-density Protein Microarray
Type 1 diabetes mellitus (T1DM) results from immune mediated destruction of pancreatic beta cells. However, clinical and immunologic phenotypes of T1DM are variable. Several auto-antibodies including GADA, IA-2A, and ZnT8A, were identified in T1DM, but the prevalence of these auto-antibodies varied ...
ORGANISM(S): Homo sapiens 
2014-07-30 | E-GEOD-50866 | biostudies-arrayexpress
ProteomicsExpanding the substantial interactome of NEMO using protein microarrays
Signal transduction by the NF-kappaB pathway is a key regulator of a host of cellular responses to extracellular and intracellular messages. The NEMO adaptor protein lies at the top of this pathway and serves as a molecular conduit, connecting signals transmitted from upstream sensors to the downstr...
ORGANISM(S): Homo sapiens 
2010-06-19 | E-GEOD-19841 | biostudies-arrayexpress
ProteomicsMass spectrometry distinguishes putrescine, spermidine, and spermine covalently attached to proteins
Bacterial and mammalian cells are rich in putrescine, spermidine and spermine. Polyamines can be incorporated into proteins in vitro. Very few naturally occurring polyaminated proteins have been identified. Bovine albumin and the recombinant universal stress protein from Francisella tularensis we...
ORGANISM(S): Bos taurus (Bovine) 
2024-02-27 | PXD046396 | Pride
Polyamine Universal stress protein Transglutaminase Mass spectrometry Manual evaluation Carbodiimide Albumin 
ProteomicsA protein microarray-based analysis of S-nitrosylation
Yeast protein microarrays were utilized to investigate determinants of S-nitrosylation by biologically relevant low-mass S-nitrosothiols (SNOs). Large numbers of S-nitrosylated yeast proteins were identified after treatment with SNOs, among which those with active-site Cys thiols residing at N termi...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-19 | E-GEOD-19218 | biostudies-arrayexpress
ProteomicsProfiling of antibodies against prostate cancer xenograft-released proteins
The present study describes a novel xenograft-based biomarker discovery platform and proves its usefulness in the discovery of novel serum markers for prostate cancer (PCa). By immunizing immuno-competent mice with serum from nude mice bearing PCa xenografts, an antibody response against xenograft-d...
ORGANISM(S): Mus musculus 
2011-12-16 | E-GEOD-16220 | biostudies-arrayexpress
ProteomicsQuantitative proteomic comparison between wild type Col and acinus-2 pinin-1 mutant using 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030081 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Pinin 
ProteomicsQuantitative proteomic comparison between wild type Col and sec-5 mutant using 15N metabolic labeling
SECRET AGENT (SEC) is an O-GlcNAc transferase involved in O-GlcNAcylation of many proteins. However, sec-5 null allele mutant phenotype is very subtle. To examine if there are proteins that show altered expression in the sec-5 mutant compared to wild-type Col, we designed MS experiments to identify ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030575 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Secret agent (sec) 
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