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MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
TranscriptomicsProteinase K treatment of Q and NQ cells
Cells in glucose-limited Saccharomyces cerevisiae cultures differentiate into quiescent (Q) and non-quiescent (NQ) fractions prior to entering stationary phase. To identify genes involved in this differentiation, Q and NQ cells from 101 deletion-mutant strains were tested for viability and reproduc...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-27 | E-GEOD-8558 | biostudies-arrayexpress
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
GenomicsProteinase-mediated macrophage signalling in psoriatic arthritis
Proteinase-mediated macrophage signalling in psoriatic arthritis
PRJNA678527 | ENA
ProteomicsProteinase K digestion of Annexin A11 fibrils
Human Annexin A11 (Uniprot accession no. P50995) head domain (residues 2-196) was recombinantly expressed, purified, fibrilized, and digested by proteinase K. Products were analyzed by LC/MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-01-20 | MSV000091107 | MassIVE
GenomicsProteinase 3 Drives Murine Diabetic Kidney Disease by Mediating Caspase-3-dependent Apoptosis of Podocytes
Proteinase 3 Drives Murine Diabetic Kidney Disease by Mediating Caspase-3-dependent Apoptosis of Podocytes
PRJNA910566 | ENA
UnknownMapping of the feline calicivirus proteinase responsible for autocatalytic processing of the nonstructural polyprotein and identification of a stable proteinase-polymerase precursor protein.
Not available
S-EPMC112747 | biostudies-literature
TranscriptomicsThe global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA)
Abstract of associated menuscript; Quinones and alpha,beta-unsaturated carbonyls are naturally occurring electrophiles that target cysteine residues via thiol-(S)-alkylation. We analyzed the global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA...
ORGANISM(S): Bacillus subtilis 
2010-05-15 | E-GEOD-13925 | biostudies-arrayexpress
UnknownDeubiquitinating function of adenovirus proteinase.
Not available
S-EPMC136223 | biostudies-literature
UnknownProteinase-Mediated Macrophage Signaling in Psoriatic Arthritis.
Not available
S-EPMC7982406 | biostudies-literature
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