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MetabolomicsSystem-wide analysis of short-term response to high temperature in Pinus radiata

Pinus radiata seedlings, the most widely planted pine species in the world, were exposed to temperatures within a range mimicking future scenarios based on current models of heat increase. The short-term heat response in P. radiata was studied in detail by exploring the metabolo...

2023-08-14 | MTBLS4567 | MetaboLights
MetabolomicsProteome allocations change linearly with the specific growth rate of Saccharomyces cerevisiae under glucose limitation

Saccharomyces cerevisiae is a widely used cell factory; therefore, it is important to understand how it organizes key functional parts when cultured under different conditions. Here, we perform a multiomics analysis of S. cerevisiae by culturing the strain with a wide range of s...

2022-08-31 | MTBLS697 | MetaboLights
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - pSNAP evaluation
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2023-02-07 | PXD024078 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – IP input
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024079 | JPOST Repository
TranscriptomicsGenome-wide search for novel human uORFs and N-terminal protein extensions using ribosomal footprinting
So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells to generate a transcriptome-wide map of TISs in a human monocytic cell line. A neural network was tr...
ORGANISM(S): Homo sapiens 
2012-08-08 | E-GEOD-39561 | biostudies-arrayexpress
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD024104 | JPOST Repository
ProteomicsSubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
We have devised a simple and robust LC-MS/MS compatible protocol for subcellular protein localization studies. Here, we combine it with in depth proteome profiling and utilize the resulting dataset to predict the subcellular localization of more than 12000 proteins in 5 human cancer cell lines. The ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-17 | MSV000086899 | MassIVE
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – LPS stimulation to RAW264.7 macrophages
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD031475 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron DIV5 vs DIV14
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2022-05-28 | PXD026346 | JPOST Repository
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