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MetabolomicsAdvanced LC-IMS-MS Protocol for Holistic Metabolite Analysis in Wine and Grape Samples
The final aim of metabolomics is the comprehensive and holistic study of the metabolome in biological samples. Therefore, the use of instruments that enable the analysis of metabolites belonging to various chemical classes in a wide range of concentrations is essential, without compromising on robus...
2026-09-12 | MTBLS8329 | MetaboLights
MetabolomicsAn LCMS-based untargeted metabolomics protocol for cochlear perilymph: highlighting metabolic effects of hydrogen gas on the inner ear of noise exposed Guinea pigs

INTRODUCTION: Noise-induced hearing loss (NIHL) is an increasing problem in society and accounts for a third of all cases of acquired hearing loss. NIHL is caused by formation of reactive oxygen species (ROS) in the cochlea causing oxidative stress. Hydrogen gas (H2) ...

2021-10-06 | MTBLS2418 | MetaboLights
MetabolomicsA robust intracellular metabolite extraction protocol for human neutrophil metabolic profiling
BACKGROUND: Neutrophils are phagocytic innate immune cells that play essential roles in host defence, but are also implicated in inflammatory diseases such as rheumatoid arthritis (RA) where they contribute to systemic inflammation and joint damage. Transcriptomic analysis of neutrophils has reveale...
2018-12-06 | MTBLS658 | MetaboLights
MetabolomicsEvaluation of specific concentrations for use in experimental protocol
Not available
ORGANISM(S): Homo sapiens 
ST000586 | MetabolomicsWorkbench
MetabolomicsLC-MS for Predator Cues Target Signaling Pathways in Toxic Algal Metabolome Protocol
Not available
ORGANISM(S): Alexandrium minutum 
ST001882 | MetabolomicsWorkbench
ProteomicsAre These Cardiomyocytes? Protocol Development Reveals Impact of Sample Preparation on the Accuracy of Identifying Cardiomyocytes by Flow Cytometry
Several protocols now support efficient differentiation of human pluripotent stem cells to cardiomyocytes (hPSC-CMs) but these still indicate line-to-line variability. As the number of studies implementing this technology expands, accurate assessment of cell identity is paramount to well-defined stu...
ORGANISM(S): Homo Sapiens 
PXD014971 | panorama
TranscriptomicsImpact of library preparation on downstream analysis and interpretation of RNA-seq data: comparison between Illumina PolyA and NuGEN Ovation protocol
Objectives: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses ...
ORGANISM(S): Homo sapiens 
2013-08-28 | E-GEOD-47933 | biostudies-arrayexpress
GenomicsWGA protocol optimization
WGA protocol optimization
PRJEB34940 | ENA
TranscriptomicsSequencing random oligonucleotides using standard GA II protocol
Study on sequencing biases introduced by library preparation step, namely by ligases. Comparison between standard Illumina protocol and improved High Definition (HD) protocol. Four replicates for N21 (21 random nucleotides), one replicate for N9 (9 random nucleotides), using either standard Illumina...
ORGANISM(S): synthetic construct 
2012-07-23 | E-GEOD-36540 | biostudies-arrayexpress
ProteomicsA novel integrated extraction protocol for multi-omic studies in heavily degraded samples
The combination of multi-omic techniques, e.g. genomics, transcriptomics, proteomics, metabolomics and epigenomics has revolutionised studies in medical research. These are employed to support biomarker discovery, better understand molecular pathways and identify novel drug targets. Despite concerte...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2024-08-09 | PXD047606 | Pride
Integrated protocol Multi-omics Ancient dna Extraction protocol Pig brain Degraded omics 
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