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We performed ChIP-chip of Loc1, Puf6, and Khd1 in order to determine the genetic loci they bind. We repeated these experiments with lighter fixation in the presence and absence of RNase to determine whether these associations are via DNA or RNA (analysis shows that they associate with the loci via ...
ORGANISM(S): Saccharomyces cerevisiae 
Chemical cross-linking coupled to mass spectrometry was used to study the complex between the nucleolar factor, Puf6, and the yeast 60S ribosome. Disuccinimidyl suberate (DSS) was used as the cross-linking reagent.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-28 | PXD024131 | Pride
Puf6 primes nuclear export of 60S pre-ribosomes at low temperature
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Saccharomyces cerevisiae 
Puf6 contributes to biogenesis of the large subunit in yeast. UV-crosslinking (CRAC) was used to determine binding sites of Puf6 on RNA.
ORGANISM(S): Saccharomyces cerevisiae 
2021-06-25 | GSE174587 | GEO
We performed ChIP-chip of Loc1, Puf6, and Khd1 in order to determine the genetic loci they bind. We repeated these experiments with lighter fixation in the presence and absence of RNase to determine whether these associations are via DNA or RNA (analysis shows that they associate with the loci via t...
ORGANISM(S): Saccharomyces cerevisiae 
2014-01-01 | GSE34245 | GEO
Genomic analyses of Loc1, Puf6, and Khd1 binding patterns and transcriptional effects
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