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ProteomicsManassantin A SILAC-Pulse Proteolysis
Manassantin A is a natural product that has been isolated from the perennial herb Saururus chinensis Baill and the aquatic plant Saururus cernuus. Manassantin A has been shown to possess potent hypoxia inducible factor 1 alpha (HIF-1α) inhibitory activity in a cell-based assay screen of thousands o...
ORGANISM(S): Homo sapiens (Human) 
2016-07-19 | PXD002305 | Pride
Drug mode-of-action In-gel digestion Mda-mb-231 Pulse proteolysis Stable isotope labeling of amino acids in cell culture Manassantin a 
ProteomicsAnalysis of newly synthesized proteins more sensitive to Proteinase K using pulse SILAC and control sample for pulse SILAC experiment in which yeast cells were heat shock for 15 min and supernatant fractions analyzed (Experiment 1)
Accurate and efficient folding of nascent protein sequences into their native state requires support from the protein homeostasis network. Herein we probed which newly translated proteins are thermo-sensitive to infer which polypeptides require more time to fold within the proteome. Specifically, we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-10 | PXD028934 | Pride
Protein homeostasis Yeast Pulse silac Limited proteolysis 
ProteomicsThe Hsp70 Chaperone System Stabilizes a Thermo-sensitive Subproteome
Stress-inducible molecular chaperones have essential roles in maintaining protein homeostasis, but the extent to which they affect global proteome stability remains unclear. Here, we analyzed the effects of the DnaK (Hsp70) system on protein stability in Escherichia coli using pulse proteolysis comb...
ORGANISM(S): Escherichia coli 
2019-06-24 | PXD012929 | Pride
Protein stability Proteomics Pulse proteolysis Chaperones 
TranscriptomicsTime-of-day-dependent light-induction of gene expression in the chicken pineal gland
Light has a strong effect on whole organism physiology, such as the circadian rhythms that are phase delayed and advanced by light given at early and late subjective night, respectively. Despite the importance of the phase-dependent light responses, little is known about the underlying molecular mec...
ORGANISM(S): Gallus gallus 
2011-03-08 | E-GEOD-21915 | biostudies-arrayexpress
UnknownChemo-Selection Strategy for Limited Proteolysis Experiments on the Proteomic Scale.
Not available
S-EPMC6524534 | biostudies-literature
UnknownProteolysis of SNARE proteins alters facilitation and depression in a specific way.
Not available
S-EPMC549003 | biostudies-literature
ProteomicsLimited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells
To study protein structures directly within cells, we developed in-cell limited proteolysis-coupled mass spectrometry (in-cell LiP-MS). Conditions for introduction of proteinase K into human cells using electroporation were optimized and validated for intracellular cleavages. In-cell LiP-MS captured...
ORGANISM(S): Homo sapiens (Human) 
2025-11-09 | PXD069095 | Pride
Stress granules Mass spectrometry In-cell lip Structural proteomics Limited proteolysis Arsenite treatment Rapamycin 
UnknownThe Copper Efflux Regulator CueR Is Subject to ATP-Dependent Proteolysis in Escherichia coli.
Not available
S-EPMC5329002 | biostudies-literature
ProteomicsUnveiling Protein Structure under Pressure on the Proteome Scale
Investigating protein structure, conformation, and activity under hydrostatic pressure is a critical challenge, particularly since a sizable portion of life is marine or deep subsurface. There is a perception that most proteins experience limited changes under pressures environmentally relevant on E...
ORGANISM(S): Thermus thermophilus HB27 
2024-09-12 | PXD047671 | Pride
High pressure Thermus thermophilus Limited proteolysis Pulse proteolysis Bottom up proteomics Extremophiles Structural proteomics 
UnknownClp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis.
Not available
S-EPMC2223743 | biostudies-literature
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