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TranscriptomicsGenome-wide CRISPR knockout screen in Huh7.5.1 human hepatocytes for the identification of host factors of hepatitis A virus
A knockout cell library in Huh7.5.1 cells was generated by introducing a genome-scale CRISPR library (GeCKOv2, Addgene #1000000049) and subjected to hepatitis A virus infection (HM175/18f) to isolate virus-resistant mutant cells. Genomic DNA was isolated from the original and virus-selected mutant c...
ORGANISM(S): Homo sapiens 
2020-01-19 | E-MTAB-8646 | biostudies-arrayexpress
TranscriptomicsFunctional genomic screens in human cells to identify host factors for SARS-CoV-2 and common cold coronaviruses
We performed genome-wide CRISPR KO screens in human Huh7.5.1 cells to select for mutations that render host cells resistant to viral infection by SARS-CoV-2, human coronavirus 229E and OC43.
ORGANISM(S): Homo sapiens 
2020-10-16 | E-MTAB-9638 | biostudies-arrayexpress
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