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MetabolomicsSelective galactose culture condition reveals distinct metabolic signatures in pyruvate dehydrogenase and complex I deficient human skin fibroblasts

INTRODUCTION: A decline in mitochondrial function represents a key factor of a large number of inborn errors of metabolism, which lead to an extremely heterogeneous group of disorders.

OBJECTIVES: To gain insight into the biochemical consequences of mitocho...

2021-10-06 | MTBLS761 | MetaboLights
MetabolomicsMitochondrial KMT9 methylates DLAT to control pyruvate dehydrogenase activity and prostate cancer growth

Prostate cancer (PCa) growth depends on de novo lipogenesis controlled by the mitochondrial pyruvate dehydrogenase complex (PDC). In this study, we identified lysine methyltransferase (KMT)9 as a novel regulator of PDC activity. KMT9 is localized in mitochondria of PCa cells, but not in mitochond...

2024-12-10 | MTBLS2452 | MetaboLights
MetabolomicsABHD11/ROLIP maintains the TCA cycle by preserving functional lipoylation of the 2-oxoglutarate dehydrogenase complex
2-oxoglutarate (2-OG) relates mitochondrial metabolism to cell function: conversion of 2-OG to succinyl-CoA by the 2-OG dehydrogenase complex (OGDHc) is rate-limiting for the Tricarboxylic Acid (TCA) cycle, but 2-OG is also required for oxygen sensitive enzymes (2-OG dependent dioxygenases) that hav...
2020-08-10 | MTBLS1875 | MetaboLights
OtherSirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Data from ProteomeXchange, PXD ID: PXD001447. Experiment: SIRT4_MITO_MOCK1, file: SIRT4_MITO_MOCK1_05.mzml. Published as part of Cell. 2014 Dec 18;159(7):1615-25 . From the Abstract: {{i}} ... Here, we establish SIRT4 as a cellular lipoamidase that regulates the pyruvate dehydrogenase complex (PDH)...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320006423 | GPMDB
Resource Reanalysis 
MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
TranscriptomicsEngineering acetyl-CoA supply: Functional expression of a bacterial pyruvate-dehydrogenase complex in the cytosol of Saccharomyces cerevisiae
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl-CoA is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into Saccharo...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-04 | E-GEOD-59814 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
MetabolomicsSARS-CoV-2 ORF7a Drives Mitochondrial Dysfunction via PDK4 Activation and Complex I Inhibition

SARS-CoV-2 reprograms host metabolism to promote viral replication and evade immune responses. While infection is known to impair mitochondrial function and enhance glycolysis, the role of viral accessory proteins in these alterations remains unclear. Here, we investigate the metabolic impact of ...

2026-07-09 | MTBLS13338 | MetaboLights
MetabolomicsPyruvate metabolism enzyme Dlat induces mitochondria protein hyperacetylation to limit fatty acid oxidation in the HFpEF heart

Increased protein acetylation is frequently observed in the failing heart, including in hearts with heart failure with preserved ejection fraction (HFpEF). However, the role of protein acetylation in cardiac metabolic impairments during the pathogenesis of HFpEF remains insufficiently investigate...

2025-09-01 | MTBLS12919 | MetaboLights
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