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TranscriptomicsTranscription profiling of human Burkittas lymphoma cell line, P493-6 to identify hypoxia responsive genes
Activation of glycolytic genes by HIF-1 is considered critical for metabolic adaptation to hypoxia. We found that HIF-1 also actively suppresses glucose metabolism through the tricarboxylic acid cycle (TCA) by directly trans-activating the gene encoding pyruvate dehydrogenase kinase 1 (PDK1). PDK1...
ORGANISM(S): Homo sapiens 
2008-06-12 | E-GEOD-4086 | biostudies-arrayexpress
Proteomicspyruvate dehydrogenase kinase 4 regulate mitochondrial dynamics
Mitochondrial dynamics provides cells with the flexibility required to adapt and respond to mitochondrial toxins, yet the mechanisms underpinning the regulation of mitochondrial dynamics machinery by these stimuli is poorly understood. Here we show that pyruvate dehydrogenase kinase 4 (PDK4) is requ...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-10 | PXD028379 | Pride
Pyruvate dehydrogenase kinase 4 Silac Mitochondrial dynamics Drp1 
TranscriptomicsEngineering acetyl-CoA supply: Functional expression of a bacterial pyruvate-dehydrogenase complex in the cytosol of Saccharomyces cerevisiae
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl-CoA is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into Saccharo...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-04 | E-GEOD-59814 | biostudies-arrayexpress
GenomicsThe impact of Pyruvate dehydrogenase kinase 4 knockout on the function of vascular smooth muscle cells
The impact of Pyruvate dehydrogenase kinase 4 knockout on the function of vascular smooth muscle cells
PRJNA1304440 | ENA
GenomicsThe impact of Pyruvate dehydrogenase kinase 4 overexpression on the function of vascular smooth muscle cells
The impact of Pyruvate dehydrogenase kinase 4 overexpression on the function of vascular smooth muscle cells
PRJNA1122490 | ENA
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
TranscriptomicsDisturbed Hepatic Carbohydrate Management During High Metabolic Demand in Medium-Chain Acyl-CoA Dehydrogenase (MCAD)-deficient Mice
Medium-chain acyl-coenzyme A (CoA) dehydrogenase (MCAD) catalyzes crucial steps in mitochondrial fatty acid oxidation, a process that is of key relevance for maintenance of energy homeostasis, especially during high metabolic demand. To gain insight into the metabolic consequences of MCAD defici...
ORGANISM(S): Mus musculus 
2012-04-25 | E-GEOD-37546 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
MetabolomicsSARS-CoV-2 ORF7a Drives Mitochondrial Dysfunction via PDK4 Activation and Complex I Inhibition

SARS-CoV-2 reprograms host metabolism to promote viral replication and evade immune responses. While infection is known to impair mitochondrial function and enhance glycolysis, the role of viral accessory proteins in these alterations remains unclear. Here, we investigate the metabolic impact of ...

2026-07-09 | MTBLS13338 | MetaboLights
TranscriptomicsThe impact of Pyruvate dehydrogenase kinase 4 knockout on the function of vascular smooth muscle cells
This study investigated the impact of pyruvate dehydrogenase kinase 4 knockout on the function of vascular smooth muscle cells.
ORGANISM(S): Mus musculus 
2026-02-22 | GSE305105 | GEO
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