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TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3513 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3516 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part 3
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RN...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3507 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters and used to valdiate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-68 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-69 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of brains, hindlimbs and tails of Xenopus laevis tadpoles after treatment with thyroid hormone
Premetamorphic Xenopus laevis tadpole tail respond to thyroid hormone by resorption. The goal of this experiment is to identify the genes involved in the TH-induced resorption tadpole tail and compare it to TH-induced proliferation and differentiation program in tadpole limb and brain. Xenopus tadpo...
ORGANISM(S): Xenopus laevis 
2006-11-08 | E-MNIA-94 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells on a novel microarray design to validate the system
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-66 | biostudies-arrayexpress
UnknownRandomized phase II study of carboplatin and paclitaxel with either linifanib or placebo for advanced nonsquamous non-small-cell lung cancer.
Not available
S-EPMC5478045 | biostudies-literature
TranscriptomicsTranscription profiling of E12.5 mouse whole embryo and E12.5 placenta total RNA pooled to create 25:75, 50:50, and 75:25 ratio mixtures to estimate transcript copy number
E12.5 mouse whole embryo and E12.5 placenta total RNA were pooled to create 25:75, 50:50, and 75:25 ratio mixtures, based on Bioanalyzer quantitation. These samples, along with the original unmixed RNAs, were used as templates for duplicate linear amplification labeling reactions. cRNA target mixtu...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-67 | biostudies-arrayexpress
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