Mouse glomeruli and brain capillary fragments were prepared. Podocytes were separated from isolated glomeruli from 8-day-old Podocin-Cre, Z/EG double transgenic mice as follows: Isolated glomeruli were incubated with trypsin solution containing 0.2 % trypsin-EDTA (Sigma-Aldrich), 100ug/ml Heparin an...
ORGANISM(S): Mus musculus
