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GenomicsMapping and dynamics of regulatory DNA in A. thaliana accessions
We performed DNase-seq on 7-day-old seedlings from four A. thaliana accessions: Bur-0, Tsu-0, Bay-0, Est-1 using INTACT constructs (Deal and Henikoff, Developmental Cell, 2010) driven by the UBQ10 promoter. Chromatin accessibility profiling (Dnase I-seq) of 7-day-old seedlings of A. thaliana accessi...
ORGANISM(S): Arabidopsis thaliana 
2013-12-15 | E-GEOD-53323 | biostudies-arrayexpress
GenomicsMapping and dynamics of regulatory DNA in A. thaliana tissues and cell types across developmental transitions
We performed DNase-seq on root tissue, root epidermal cell types, seed coat epidermal cells at two developmental stages, and open and closed flowers. Root: We performed chromatin profiling (DNase-seq) on whole root (all cell types), or root epidermal cell types (hair cell, non-hair cell). Seed coat:...
ORGANISM(S): Arabidopsis thaliana 
2013-12-15 | E-GEOD-53324 | biostudies-arrayexpress
TranscriptomicsMapping and dynamics of regulatory DNA and transcription factor networks in A. thaliana
We mapped DNaseI hypersensitive sites (DHSs) and applied genomic footprinting to define in vivo transcription factor (TF) occupancy at nucleotide resolution across the A. thaliana genome in whole seedlings during heat- and light-response states. We find that trait-associated variation localizes with...
ORGANISM(S): Arabidopsis thaliana 
2013-12-15 | E-GEOD-53322 | biostudies-arrayexpress
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