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Rab35 restrains regional Wnt activation by regulating a Cdc42-JNK signalling relay in the intestine
Rab35 knock-down during in vitro hepatocyte differentiation
The hippocampi from the control (Rab35flox/+; Nestin-Cre) and Rab35 cKO mice groups at P0 (n = 5 biological replicates per genotype) were lysed in a buffer containing 6 M guanidine hydrochloride, 100 mM HEPES-NaOH (pH 8.0), 10 mM TCEP, and 40 mM chloroacetamide. The lysates were dissolved through he...
ORGANISM(S): Mus Musculus (mouse) 
We performed siRNA-mediated knockdown of Rab35 GTPase in primary mouse hepatoblasts during their differentiation into hepatocytes and analyzed changes in gene expression profiles in cells treated with Rab35 or control (Luciferase) siRNA at the final day of the culture.
ORGANISM(S): Mus musculus 
2024-10-01 | GSE271270 | GEO
Effect of Rab35 knockdown on in vitro differentiated hepatocyte gene expression (time-course)
Rab35 knockdown results in a loss of apical bulkheads from hepatocytes, which causes isotropic growth of the bile canaliculus (BC) and ultimately results in the formation of hepatocyte cysts. In these cysts, hepatocytes have common apico-basal polarity, such as observed for simple epithelia like cho...
ORGANISM(S): Mus musculus 
2024-10-16 | GSE209876 | GEO
To study the role of Rab35 in the formation of bile canaliculi, primary mouse hepatoblasts were acquired, cultured in matrigel, and subjected to Rab35 knockdown by siRNA with scrambled control (4 biol. replicates each, block-randomised). Samples were subjected to S-trap protein digest and label-free...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-03-06 | MSV000091413 | MassIVE
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