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GenomicsZEB1 ChIP-seq Data from Control CD8+cDC1
To identify the direct targets of Zeb1 we performed ChIP-seq of wild type cDC1 cell line in unstimulated condition. cDC1 cell line was used for Chromatin Immunoprecipitation, it was then fixed and crosslinked and then fragmented and the fragmented DNA-protein was immunoprecipated using Zeb1 antibod...
ORGANISM(S): Mus musculus 
2018-11-13 | E-MTAB-6917 | biostudies-arrayexpress
TranscriptomicsRNA-seq from ZEB1 Knockdown and Control CD8+cDC1
To have a mechanistic insight how the Zeb1 KD CD8+cDC1 perturbs the immune response globally. to identify the genes that are regulated by Zeb1 RNA was isolated and check for quality, then we used NEB RNA library preparation kit to prepare the library and send for sequencing on Illumina Hi-seq 2500 p...
ORGANISM(S): Mus musculus 
2018-11-13 | E-MTAB-6916 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation
RNA-seq was performed to understand the role of NCoR1 in CD8α+ DCs. MutuDC cell line was transduced with empty and Ncor1 shrna lentiviral particles and cells were prepared in unstimulated, 2hr and 6hr (IFNg, pIC and CpG+pIC+IFNg) stimulation condition.
ORGANISM(S): Mus musculus 
2022-09-20 | E-MTAB-10989 | biostudies-arrayexpress
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