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GenomicsZEB1 ChIP-seq Data from Control CD8+cDC1
To identify the direct targets of Zeb1 we performed ChIP-seq of wild type cDC1 cell line in unstimulated condition. cDC1 cell line was used for Chromatin Immunoprecipitation, it was then fixed and crosslinked and then fragmented and the fragmented DNA-protein was immunoprecipated using Zeb1 antibod...
ORGANISM(S): Mus musculus 
2018-11-13 | E-MTAB-6917 | biostudies-arrayexpress
TranscriptomicsRNA-seq from ZEB1 Knockdown and Control CD8+cDC1
To have a mechanistic insight how the Zeb1 KD CD8+cDC1 perturbs the immune response globally. to identify the genes that are regulated by Zeb1 RNA was isolated and check for quality, then we used NEB RNA library preparation kit to prepare the library and send for sequencing on Illumina Hi-seq 2500 p...
ORGANISM(S): Mus musculus 
2018-11-13 | E-MTAB-6916 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation
RNA-seq was performed to understand the role of NCoR1 in CD8α+ DCs. MutuDC cell line was transduced with empty and Ncor1 shrna lentiviral particles and cells were prepared in unstimulated, 2hr and 6hr (IFNg, pIC and CpG+pIC+IFNg) stimulation condition.
ORGANISM(S): Mus musculus 
2022-09-20 | E-MTAB-10989 | biostudies-arrayexpress
GenomicsZEB1 in 3T3-L1 differentiation (ChIP-seq)
We aimed to characterise genome-wide ZEB1 binding pattern in 3T3-L1 cells at distinct timepoints of adipogenic differentiation (days -2, 0, 2 and 4). We performed replicate experiments at days -2 and 0 and single experiments at days 2 and 4.
ORGANISM(S): Mus musculus 
2014-09-17 | E-MTAB-2537 | biostudies-arrayexpress
GenomicsChIP-seq of H3K27ac in Control and NCoR1 KD CD8α+ DCs at 0hr, 2hr and 6hr of TLR stimulation
H3K27ac ChIP-seq was performed to identify active enhancers in different TLR activated control CD8α+ DCs as well as to understand the role of NCoR1 in regulating TLR specific enhacner activity using H3K27ac ChIP-seq performed in NCoR1 KD DCs. MutuDC1940 cell line were treated with TLR3 ligand poly I...
ORGANISM(S): Mus musculus 
2025-12-03 | E-MTAB-10992 | biostudies-arrayexpress
TranscriptomicsSMRT controlled inflammation in cDC1 is orchestrated through mTOR-Succinate axis despite of suppressed glycolysis
Immunogenic activation of DCs by TLRs involves coordinated metabolic and transcriptional rewiring. The negative regulation of such metabolic rearrangements in DCs remains elusive. We have recently reported that Ncor2 (SMRT) loss of function renders cDC1 DCs to be highly inflammatory in nature by pol...
ORGANISM(S): Mus musculus 
2024-10-01 | E-MTAB-13410 | biostudies-arrayexpress
GenomicsNCoR1, SMRT, Polymerase II and input ChIP-Seq
ChIP-seq of mouse embryonic fibroblast-adipose like cell line 3T3-L1 to identify binding sites of NCoR1 and SMRT following induction of differentiation, and RNA Pol-II after SMRT knock down
ORGANISM(S): Mus musculus 
2012-04-23 | E-MTAB-1031 | biostudies-arrayexpress
TranscriptomicsZEB1 in 3T3-L1 differentiation (RNA-seq)
We aimed to characterise genome-wide gene expression changes upon ZEB1 knockdown in 3T3-L1 cells at distinct timepoints of adipogenic differentiation (days 0 and 2), performing replicate experiments.
ORGANISM(S): Mus musculus 
2014-09-17 | E-MTAB-2538 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of early transcriptional events induced by cytokine IL-4 in human cord blood CD4+ T cells
The aim of this dataset was to study in detail the transcription kinetics initiated by cytokine IL-4 in early differentiation of Th2 cells. Total RNA obtained from activated and IL-4 & anti-IL-12 treated cord blood CD4+ T cells, 0.5, 1, 2, 4, 6, 12, 24, 48, and 72 hours after initiation of the cultu...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-17974 | biostudies-arrayexpress
GenomicsGenome-wide detection of STAT6 binding sites in IL-4 treated naive human CD4+ T cells
STAT6 is a major transcription factor driving the polarization of Th2 cells in response to IL-4. STAT6 is phosphorylated by Jak1 and Jak3 kinases at the IL-4 receptor, after which phosphorylated STAT6 forms a homodimer and translocates into the nucleus. There STAT6 binds to specific DNA sequences, r...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-17850 | biostudies-arrayexpress
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