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UnknownA Triple Knockout Isobaric-Labeling Quality Control Platform with an Integrated Online Database Search.
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S-EPMC7332369 | biostudies-literature
ProteomicsA Triple KnockOut Isobaric-labelling Quality Control Platform in an Integrated On-line Database Search
Sample multiplexing using isobaric tagging is a powerful strategy for proteome-wide protein quantification. One major caveat of isobaric tagging is ratio compression that results from the isolation, fragmentation, and quantification of co-eluting, near-isobaric peptides, a phenomenon typically refer...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-31 | PXD017803 | Pride
Tko standard  ion interference  tmt  itraq  sps-ms3  tvt  shiny application 
ProteomicsA mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides
Less than half of all MS/MS spectra acquired in shotgun proteomics typically result in a confident peptide match. Here we present an ultra-tolerant Sequest database search that allowed peptide matching even with modifications of unknown masses up to ±500 Da. From an HEK293 cell proteome-wide datas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-06-19 | MSV000079841 | MassIVE
ProteomicsBioplex 3 AP-MS Dataset for Library Construction
The BioPlex (biophysical interactions of ORFeome-based complexes) network is the result of creating thousands of cell lines with each expressing a tagged version of a protein from the ORFeome collection. Immunopurification of the tagged protein and detection of associated proteins by mass spectromet...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-12-13 | MSV000088555 | MassIVE
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