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ProteomicsComparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm
The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore,...
ORGANISM(S): Bombyx mori (Silk moth) 
2024-05-24 | PXD042494 | Pride
Real-time pcr Model cookies Alpha-amlyase Lipoprotein Bombyx mori Silkworm Lc-ms/ms 
TranscriptomicsReal-time PCR analysis of microRNA expression in differentiating human skeletal muscle cells
Human skeletal myoblast cell line LHCN-M2 (Zhu et al., Aging Cell (2007), vol. 6, pp 515-523) were plated on collagen-coated 6-well Falcon cell culture plates at 500 000 cells/well, differentiation was induced 24h later by switching to DMEM supplemented with 0,01 mg/ml insulin and 0,1 mg/ml transfer...
ORGANISM(S): Homo sapiens 
2013-04-11 | E-GEOD-45957 | biostudies-arrayexpress
TranscriptomicsIdentification of reference genes for quantitative Real-Time PCR in Bifidobacterium bifidum PRL2010 based on transcriptome analysis
In this study, we investigated the transcriptome of Bifidobacterium bifidum PRL2010 during in vitro growth by micro array technology. When B. bifidum PRL2010 was grown in liquid broth, 425 of the 1644 PRL2010 genes represented on the array were expressed in at least one of the three investigated gro...
ORGANISM(S): Bifidobacterium bifidum 
2012-01-05 | E-GEOD-30832 | biostudies-arrayexpress
GenomicsReal-time quantitative PCR analysis of microRNAs in serum
Real-time quantitative PCR analysis of microRNAs in serum
PRJNA832567 | ENA
TranscriptomicsReal-time quantitative PCR analysis of human astrocytomas
Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not children. We examined the global protein and microRNAs expression pattern by 2D SDS-PAGE, mass spect...
ORGANISM(S): Homo sapiens 
2013-02-12 | E-GEOD-44280 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation
Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI ...
ORGANISM(S): Homo sapiens 
2014-06-01 | E-GEOD-51677 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 2 week old male MeCP2 A140V mutant mouse cerebellum
The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates. The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 2 ...
ORGANISM(S): Mus musculus 
2015-02-01 | E-GEOD-35690 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 4 week old male MeCP2 A140V mutant mouse cerebellum
The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 4 w...
ORGANISM(S): Mus musculus 
2015-02-01 | E-GEOD-35691 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mouse immature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted immature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was pe...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49270 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 4 week-old male MeCP2 A140V mutant mouse cerebellum
The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Epigenetic Chromatin Modification Enzymes Array (Catalog# PAMM-085A) from SABiosciences. Mice used in t...
ORGANISM(S): Mus musculus 
2015-02-01 | E-GEOD-35689 | biostudies-arrayexpress
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