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TranscriptomicsLong non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression regulation. To study lncRNAs in cancer, we generated expression profiles for 1708 human lncRNAs in the NCI60 cancer cell line panel using a high-throughput nanowell RT-qPC...
ORGANISM(S): Homo sapiens 
2016-06-29 | E-GEOD-80332 | biostudies-arrayexpress
TranscriptomicsReal-time PCR analysis of microRNA expression in differentiating human skeletal muscle cells
Human skeletal myoblast cell line LHCN-M2 (Zhu et al., Aging Cell (2007), vol. 6, pp 515-523) were plated on collagen-coated 6-well Falcon cell culture plates at 500 000 cells/well, differentiation was induced 24h later by switching to DMEM supplemented with 0,01 mg/ml insulin and 0,1 mg/ml transfer...
ORGANISM(S): Homo sapiens 
2013-04-11 | E-GEOD-45957 | biostudies-arrayexpress
GenomicsReal-Time qPCR Array of miRNAs in Leishmania-infected THP-1 macrophages
Real-Time qPCR Array of miRNAs in Leishmania-infected THP-1 macrophages
PRJNA1013679 | ENA
TranscriptomicsHuman p53 signaling qPCR primer Array
H1299 cells were overexpressed miR-138 or silenced AGO2. The expression of 92 genes associated with p53 using the “Human p53 Signaling Pathway PCR Array” qPCR gene expression profiling. H1299 cells were transfected with NC mimics, AGO2 siRNA or miR-138 for 48h. Equal amount total RNA from each group...
ORGANISM(S): Homo sapiens 
2015-06-05 | E-GEOD-69562 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of microRNA expression in Primary human Bronchial Epithelial cells (PBECs) from healthy or asthmatic subjects
The goal of this study is to compare the base-line microRNA expression of asthmatic and healthy PBECs. PBECs were grown in-vitro up to passage 2 before harvest. RT-qPCR based microRNA array expression profiling. PBECs were isolated from 5 healthy and 5 asthmatic donors. Total cellular RNA from each ...
ORGANISM(S): Homo sapiens 
2014-09-19 | E-GEOD-61563 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of miRNA expression in human melanoma MMRU cell line after Sox4 knockdown
We used Applied Biological Materials (ABM)’s miRNA profiling platform to quantitate expression of miRNAs in a human melanoma cell line (MMRU) in Sox4 siRNA treated cells (siSox4) compared with the control siRNA treated cells (siCTR) or after overexpression of Flag-Dicer in Sox4 knockdown cells (siSo...
ORGANISM(S): Homo sapiens 
2012-05-31 | E-GEOD-36715 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mouse immature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted immature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was pe...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49270 | biostudies-arrayexpress
TranscriptomicsReal-time based miRNA array for MCF10 derived cell series with different metastatic potentials
MCF10AT1 (M-II), MCF10CA1h (M-III) and MCF10CA1a.cl1 (M-IV) are a set of human breast cancer cell lines that derived from the same parent cell line MCF10 but display distinct phenotypes in metastatic potential in the form of xenografts in immune-compromised mice. From M-II to M-IV, the metastatic po...
ORGANISM(S): Homo sapiens 
2012-12-13 | E-GEOD-42884 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of human malignant pleural mesothelioma tissue specimens
BACKGROUND: Malignant pleural mesothelioma (MPM) is difficult to distinguish from reactive mesothelial proliferations (RMPs). MicroRNAs (miRNAs) are small non-coding RNA-strands (~22 nucleotides) that post-transcriptionally regulate gene-expression. Studies have shown that miRNAs are potential diagn...
ORGANISM(S): Homo sapiens 
2014-01-25 | E-GEOD-54394 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mice lung tissue (1)
Recombinant-murine S100A8 and the corresponding Cys42 to Ala42 mutant were used at 10 mg/50 ml HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, 12 or 20 h post-inhalation of S100A8 or 12 and 20 h post-inhalation of Ala42S100A8. For comparison w...
ORGANISM(S): Mus musculus 
2013-11-26 | E-GEOD-52718 | biostudies-arrayexpress
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