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Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes

Genes encoding Arabidopsis subunit homologs of TFIIH kinase module were labelled with coding sequences of GFP and mCherry (green and red fluorescent proteins) using fast-track recombineering. To illustrate the applicability of recombineering for accelerating the isolation and identification of plant...

ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress)

2019-08-01 | PXD013637 | Pride

Prioritization of antimicrobial targets by CRISPR-based oligo recombineering

Nucleophilic amino acids are important in covalent drug development yet underutilized as antimicrobial targets. Over recent years, several chemoproteomic technologies have been developed to mine chemically-accessible residues via their intrinsic reactivity toward electrophilic probes. However, these...

ORGANISM(S): Toxoplasma gondii RH

2022-10-24 | PXD035658 | Pride

Toxoplasma gondii Oligo recombineering Proteomics

Recombineering and MAGE.

Not available

S-EPMC9083505 | biostudies-literature

Genetic Engineering by DNA Recombineering.

Not available

S-EPMC6727988 | biostudies-literature

High efficiency recombineering in lactic acid bacteria.

Not available

S-EPMC3378904 | biostudies-literature