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GenomicsStudy of RFX1 and RFX3 binding sites in the mouse genome
The aim of the study was to identify RFX1 and RFX2 binding sites in mouse pancreatic beta cells. Chromatin immunoprecipitation experiments were performed with the mouse MIN6 beta cell line and antibodies raised against RFX1 and RFX3. Immunoprecipitated DNA was sequenced using the Genome Analyzer II ...
ORGANISM(S): Mus musculus 
2015-08-22 | E-GEOD-72272 | biostudies-arrayexpress
TranscriptomicsCompare gene expression profile of bone-marrow derived dendritic cells from wild-type and miR155 knockout mice
Compare gene expression profile of bone-marrow derived conventional dendritic cells fom wild-type and miR155 knockout mice
ORGANISM(S): Mus musculus 
2011-11-22 | E-MTAB-497 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation
miRNA expression profiling of human monocyte-derived dendritic cells (moDCs) during maturation. Immature, 4h and 16h LPS-activated moDCs were used. In this study were analysed 2 biological samples of RNA extracted from 4h-post LPS stimulation moDCs and 2 bfrom 16h-post LPS stimulation. The RNA ...
ORGANISM(S): Homo sapiens 
2010-05-15 | E-GEOD-13369 | biostudies-arrayexpress
TranscriptomicsRepression of arginase 2 expression in dendritic cells by microRNA-155 is critical for promoting T-cell proliferation
Microarray experiments were performed to compare the gene expression profiles exhibited by immature and activated bone-marrow (BM) derived conventional DCs (BM-cDCs) and plasmacytoid DCs (BM-pDCs) from WT and miR155-/- mice. (time points 0, 4 and 24 hours)
ORGANISM(S): Mus musculus 
2015-01-09 | E-MTAB-1527 | biostudies-arrayexpress
TranscriptomicsmiR-155 KO in human dendritic cells
In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-deri...
ORGANISM(S): Homo sapiens 
2009-03-04 | E-GEOD-13296 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 30 [RNA-Seq P30]
Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: RNA was extracted from decapsulated testes of 29-30 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of th...
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-68283 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 21 [RNA-Seq P21]
Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: RNA was extracted from decapsulated testes of 21 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of the g...
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-68284 | biostudies-arrayexpress
GenomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-63130 | biostudies-arrayexpress
GenomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [ChIP-Seq]
Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: DNA was extracted from decapsulated testes of 21 day old mice. ChIP sequencing was used to determine the binding sites of RFX2. Results: ChIP-Seq analysis identified ~88...
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-68282 | biostudies-arrayexpress
TranscriptomicsMicroRNAome comparison between macrophages infected with Leishmania RNA virus containing L.guyanensis and virus-free parasites
Leishmania RNA virus is an endosymbiotic virus of obligate intracellular Leishmania parasites. The presence of Leishmania RNA virus has been associated to metastatic leishmaniasis in hamsters and the failure of the first-line treatment in humans. This experiment aims to find the differences in the m...
ORGANISM(S): Mus musculus 
2016-09-01 | E-MTAB-4413 | biostudies-arrayexpress
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