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TranscriptomicsRIP-seq of polyribosomal SLBP RNP
UV cross-linking and immunoprecipiatation with high throughput sequencing of cytoplasmic SLBP RNP Polyclonal α-SLBP antibody was used to immunoprecipitate SLBP RNP from polyribosomal fractions of HeLa S3 lysates. Mock immunoprecipitation were also performed and serve as a negative control.
ORGANISM(S): Homo sapiens 
2015-12-16 | E-GEOD-62076 | biostudies-arrayexpress
TranscriptomicsGene expression in RNP autoantibody+ systemic lupus erythematosus (SLE) patient blood
The goal of this study was to characterize gene expression profiles in RNP autoantibody+ SLE versus healthy blood donors with a focus on select cytokines that may be important in B cell activation and differentiation, including BAFF, IL-21, and IL-33. We utilized Affymetrix microarrays to characteri...
ORGANISM(S): Homo sapiens 
2015-01-01 | E-GEOD-61635 | biostudies-arrayexpress
TranscriptomicsGene expression in RNP autoantibody+ systemic lupus erythematosus (SLE) patient blood
The goal of this study was to characterize gene expression profiles in RNP autoantibody+ SLE versus healthy blood donors with a focus on select cytokines that may be important in B cell activation and differentiation, including BAFF, IL-21, and IL-33. We utilized Affymetrix microarrays to characteri...
ORGANISM(S): Homo sapiens 
2015-01-01 | GSE61635 | GEO
TranscriptomicsRIP-seq data for CCNT1
To demonstrate RIPSeeker program that is developed for RIP-seq analyses, we generated RIP-seq data corresponding to the protein CCNT1 in HEK293 cell line using standard RIP-seq protocols described in Zhao et al., (2010). We performed two in-house RIP-seq experiments both for CCNT1 in human HEK293 ce...
ORGANISM(S): Homo sapiens 
2012-12-28 | E-GEOD-43170 | biostudies-arrayexpress
UnknownUnique Interactions of the Nuclear Export Receptors TbMex67 and TbMtr2 with Components of the 5S Ribonuclear Particle in Trypanosoma brucei.
Not available
S-EPMC6695518 | biostudies-literature
TranscriptomicsIdentification of RNAs associated with the Caenorhabditis elegans spliced-leader trans-splicing proteins SNA-2, SNA-3, SNR-2 and SUT-1 proteins by RIP-Seq.
This study aimed to identify RNAs bound to the C. elegans SNA-2, SNA-3, SUT-1 and SNR-2 proteins involved in spliced leader 1 trans-splicing through use of RNA immunoprecipitation sequencing (RIP-Seq). Four IP sample replicates and four control sample replicates were sequenced for each protein.
ORGANISM(S): Caenorhabditis elegans 
2024-04-24 | E-MTAB-13193 | biostudies-arrayexpress
TranscriptomicsIdentification of microRNAs and microRNA targets in Xenopus ectoderm
Purpose: The establishment of cell lineages occurs via a dynamic progression of gene regulatory networks that underlie developmental commitment and differentiation. To investigate how microRNAs (miRs) function in this process, we compared miRs and miR targets at the initiation of the two major ectod...
ORGANISM(S): Xenopus laevis 
2016-06-28 | E-GEOD-83784 | biostudies-arrayexpress
TranscriptomicsRIP-seq from ENCODE/SUNY Albany
This data was generated by ENCODE. If you have questions about the data, contact the submitting laboratory directly (Scott Tenenbaum mailto:STenenbaum@uamail.albany.edu). If you have questions about the Genome Browser track associated with this data, contact ENCODE (mailto:genome@soe.ucsc.edu). The...
ORGANISM(S): Homo sapiens 
2012-06-06 | E-GEOD-35585 | biostudies-arrayexpress
TranscriptomicsIdentification of Neuronal RNA Targets of TDP-43-Containing Ribonucleoprotein Complexes
We identify the RNA targets of TAR DNA-binding protein 43 (TDP-43) from cortical neurons by RNA immunoprecipitation followed by deep sequencing (RIP-seq). We identify 4352 highly enriched RNA targets of TDP-43. We determined that the canonical TDP-43 binding site (TG)n was 55.1-fold enriched in our ...
ORGANISM(S): Rattus norvegicus 
2010-11-04 | E-GEOD-25032 | biostudies-arrayexpress
TranscriptomicsReduced expression of RBM47 enhances Nrf2 activity to promote tumor growth in A549 lung adenocarcinoma cells
We identified RNA binding motif protein 47 (RBM47) as a target gene of transforming growth factor (TGF)-beta in mammary gland epithelial cells (NMuMG cells) that have undergone the epithelial-to-mesenchymal transition (EMT). TGF-beta repressed RBM47 expression in NMuMG cells and lung cancer cell lin...
ORGANISM(S): Homo sapiens 
2016-03-01 | E-GEOD-61910 | biostudies-arrayexpress
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