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TranscriptomicsIdentification of KSHV, EBV and cellular miRNA targets in human B-cells using RIP-Chip
RIP-Chip was performed on DG75-eGFP, DG75-10/12, BCBL-1, BL41, BL41 B95.8 and Jijoye using anti-human Ago2 (11A9) antibodies. Anti-BrdU antibodies were used as controls for DG75-eGFP, DG75-10/12 and BCBL-1. Total RNA was used as control for BL41, BL41 B95.8 and Jijoye. Samples were analyzed on Affym...
ORGANISM(S): Homo sapiens 
2011-02-04 | E-GEOD-17180 | biostudies-arrayexpress
TranscriptomicsIdentification of miR-205 targets using an RIP-Chip assay with AGO2 antibody
In this study, the prognostic properties of miR-205 expression levels are investigated in a well-documented prostate cancer cohort. We show that miR-205 is correlated to shortened overall survival, significantly dividing the PCa patients into high and low risk groups. Furthermore, miR-205 is shown t...
ORGANISM(S): Homo sapiens 
2013-12-31 | E-GEOD-39735 | biostudies-arrayexpress
TranscriptomicsRIP-chip
Mutant genotype miR-125a precurrsor was found in recurrent pregnancy loss patients. We use RIP-chip assay to detect the disturbed mRNA profiling in RISC of HEK293T cells after wildtype or mutant genotype miR-125a expression vector transfection.
ORGANISM(S): Homo sapiens 
2015-12-01 | E-MTAB-2394 | biostudies-arrayexpress
TranscriptomicsHuR (ELAVL1) RIP-chip and knockdown exon array
Integrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA processing and mRNA stability In this dataset, we employed two distinct experiments. 1) HuR RIP-chip to identify mRNA targets of HuR. 2) HuR knockdown to identify mRNAs whose expression are dependent ...
ORGANISM(S): Homo sapiens 
2011-08-18 | E-GEOD-29778 | biostudies-arrayexpress
TranscriptomicsRIP-Chip analysis of Musashi (Msi1) in the Daoy human medulloblastoma cell line.
RIP-chip analysis to identify mRNA preferentially associated with Msi1 protein. RIP-Chip experiments were performed on two biologically replicated samples transfected with the BAP-Msi1 construct and a control sample from cells transfected with the BAP-Control construct. A total of 8 microarrays were...
ORGANISM(S): Homo sapiens 
2011-07-25 | E-GEOD-30904 | biostudies-arrayexpress
TranscriptomicsRIp-chip experiments with the Scw1 RNA-binding protein on ageing samples
We have used RIp-chip to identify mRNAs that coprecipitate with specific Scw1 RNA-binding protein on ageing samples
ORGANISM(S): Schizosaccharomyces pombe 
2019-06-01 | E-MTAB-7618 | biostudies-arrayexpress
TranscriptomicsHuR differentially regulates unique subsets of mRNAs in estrogen receptor negative and positive breast cancer
Analysis of RNA immunoprecipitation of HuR, a RNA binding protein (RBP), in breast cancer cell lines. This approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs. HuR is an RBP that binds ...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-17820 | biostudies-arrayexpress
Transcriptomicstrf4, trf5, and trf4-DADA mutant cells gene expression; Trf4p X-RIP-Chip
This SuperSeries is composed of the following subset Series: GSE16103: Wild-type versus trf4, trf5, and trf4-DADA mutant cells GSE16105: Trf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip) Refer to individual Series
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-16107 | biostudies-arrayexpress
TranscriptomicsRIp-chip experiments with the Scw1, Zfs1 and Red1 RNA-binding proteins
We have used RIp-chip to identify mRNAs that coprecipitae with specific RNA-binding proteins
ORGANISM(S): Schizosaccharomyces pombe 
2014-11-06 | E-MTAB-2709 | biostudies-arrayexpress
TranscriptomicsSpecific sequence determinants of miR-15/107 microRNA gene group targets
Using anti-Argonaute (anti-AGO) antibody co-immunoprecipitation, followed by microarray analyses and downstream bioinformatics, ‘RIP-Chip’ experiments enable direct analyses of miRNA targets. The analyses support four major findings: (i) RIP-Chip studies correlated with total input mRNA profiling pr...
ORGANISM(S): Homo sapiens 
2012-12-11 | E-GEOD-42823 | biostudies-arrayexpress
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