Sort   by:  
 Page size 
TranscriptomicsIdentification of long non-coding RNA expression patterns useful for molecular-based classification of type I endometrial cancers
Endometrial cancer represents the most frequent gynecologic malignant disease. Although several genetic alterations have been associated with increased risk, to date diagnosis and prognosis still rely on morphological features of the tumor, such as histological type, grading and invasiveness. As mol...
ORGANISM(S): Homo sapiens 
2019-04-30 | E-MTAB-7039 | biostudies-arrayexpress
TranscriptomicsTimed regulation of Piwi-interacting RNAs expression during rat liver regeneration
Small noncoding (snc) RNAs represent a growing family of transcripts that regulate key cellular processes, including mRNA degradation, translational repression and transcriptional gene silencing. Among these, the PIWI-interacting RNAs (piRNAs), a major class of sncRNAs initially identified in the ge...
ORGANISM(S): Rattus norvegicus 
2014-06-17 | E-GEOD-55783 | biostudies-arrayexpress
TranscriptomicsRNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer
PIWI-interacting RNAs (piRNAs) are a novel class of small ncRNAs initially isolated from germ line cells; although recent studies report that they are expressed also in somatic cells. To elucidate the role of piRNAs in somatic cells, in particular from breast cancer, we performed the first extensive...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-56134 | biostudies-arrayexpress
TranscriptomicsAnalysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq
Using a chemical treatment, the sodium periodate (NaIO4) /β-Elimination oxidation, and then performing deep-sequencing of small RNAs with NGS technologies, we evaluated the methylation status of the piRNAs population in the metastatic COLO 205 cell line.
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8115 | biostudies-arrayexpress
TranscriptomicsRNA-Immunoprecipitation of PIWIL1/piRNA complexes in COLO 205 cells and deep-sequencing of associated small RNAs with NGS technologies
To evaluate PIWIL1/piRNAs association in the metastatic COLO 205 cell line, we performed an RNA-immunoprecipitation experiment followed by small RNA deep-sequencing (RIP-Seq). For the identification of PIWIL1/piRNA complexes, independent reactions were carried out with two different antibodies (IP1 ...
ORGANISM(S): Homo sapiens 
2020-08-05 | E-MTAB-8121 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of total RNA in the COLO 205 cell line with Next-Generation Sequencing (NGS) technologies
The transcriptome of COLO 205 cells was investigated using an RNA-Seq approach. This allowed us to evaluate the expression of mRNAs encoding for the major interactors of PIWIL proteins and factors involved in piRNAs biogenesis.
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8116 | biostudies-arrayexpress
TranscriptomicsExpression profiles of piRNAs in Colorectal Cancer (CRC) cell lines
To investigate the presence and abundance of piRNAs in Colorectal Cancer (CRC), we performed deep-sequencing of the small RNA transcriptome of eight human CRC cell lines (HT-115, Caco-2, SW-1417, SW 403, COLO 205, HT-29, HCT 116 and RKO).
ORGANISM(S): Homo sapiens 
2020-08-06 | E-MTAB-8117 | biostudies-arrayexpress
TranscriptomicsGene expression profile in breast cancer cell lines using miRNA over-expression
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag at the C-term (C-TAP-ER-beta). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) for ...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-MTAB-1493 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile between ER-beta- and ER-beta+ breast tumors.
FFPE tissues samples were cut in 5-µm-thick sections on a microtome with a disposable blade. RNA was extracted either from three or eight sequential sections using miRNeasy FFPE Kit (QIAGEN), following the steps described in the manufacturers protocol. RNA extracted from same biological samples and...
ORGANISM(S): Homo sapiens 
2011-10-14 | E-TABM-1054 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile in breast cancer cells
We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from MCF-7 cells expressing human ER-beta in absence of estrogen stimuli was subjected to size selection labrary preparation ond retrotranscribed. Obtained DNA was sequenced with the Illumina...
ORGANISM(S): Homo sapiens 
2014-11-13 | E-MTAB-2085 | biostudies-arrayexpress
Sort   by:  
 Page size