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ProteomicsComplementary CID and 351 nm UVPD tandem mass spectra enable effective de novo sequencing
This dataset accompanies a publication in which we present a strategy for acquisition and effective de novo peptide sequencing of complementary CID and 351 nm ultraviolet photodissociation (UVPD) MS/MS pairs. E. coli whole cell lysate is carbamylated to block lysine side chains, digested with trypsi...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2017-03-06 | MSV000080603 | MassIVE
ProteomicsAMCA chromophore tagging and 351 nm ultraviolet photodissociation enables de novo sequencing of E. coli proteome
Demonstration of novel experimental and computational pipeline for high performance de novo peptide sequencing. E. coli whole cell lysate is carbamylated to block lysine side chains, digested with trypsin (now active only at Arg residues), and N-terminally tagged with the chromophore AMCA. Three tec...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2016-03-10 | MSV000079571 | MassIVE
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