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TranscriptomicsMicroarray profiling of polyploid macrophage progeny
To explore how bacterial lipoproteins (i.e. TLR2 ligands) increased macrophage ploidy via mitotic defects, we performed global analysis of cell cycle gene expression changes induced by bacterial lipoproteins. FSL-1 (Pam2CGDPKHPKSF) is a synthetic lipoprotein that represents the N-terminal part of th...
ORGANISM(S): Mus musculus 
2016-10-27 | E-MTAB-5085 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
The transcriptome of splenic classical dendritic cells (cDCs) (CD3- CD19- CD45+ MHC-II+ CD11c+) was compared in Wild Type (WT) mice, Germ Free (GF) mice and mice deficient for the Ifnar1 gene (IFNAR). Cells were isolated and sorted by flow cytometry to high purity. The cells were stimulated ex vivo ...
ORGANISM(S): Mus musculus 
2018-06-30 | E-MTAB-5190 | biostudies-arrayexpress
GenomicsChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
The epigenetic state of splenic cDCs from Wild Type (WT) mice, Germ Free (GF) and mice deficient for the Ifnar1 gene (IFNAR) was characterized by analyzing the occupancy of a major activating histone mark (H3K4me3) and a major repressive histone mark (H3K27me3). Splenic cDCs (CD3- CD19- CD45+ MHC-II...
ORGANISM(S): Mus musculus 
2018-06-30 | E-MTAB-5234 | biostudies-arrayexpress
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