Sort   by:  
 Page size 
ProteomicsRNF185 control of COL3A1 expression limits prostate cancer migration and metastatic potential.
RNF185 is a RING finger domain-containing ubiquitin ligase implicated in ER-associated degradation. Prostate tumor patient data analysis revealed a negative correlation between RNF185 expression and prostate cancer progression and metastasis. Likewise, several prostate cancer cell lines exhibited gr...
ORGANISM(S): Mus musculus (Mouse) 
2024-08-09 | PXD045473 | Pride
Ubiquitin ligase  rnf185  prostate adenocarcinoma  metastasis  epithelial-mesenchymal transition (emt)  collagen 
ProteomicsSPANX immunoprecipitation analysis by mass spectrometry
A375 cells transduced with empty pLVX or pLVX-SPANXA-FLAG were subjected to immunoprecipitation using Flag beads. Elution from beads was performed by incubation with 3XFlag peptide for 1h at 4 C and eluates were subjected to tryptic digestion followed by LC-MS/MS, as described previously (PMID: 3099...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-24 | MSV000084617 | MassIVE
ProteomicsRNF5 interactome
Cells expressing Flag-tagged RNF5 or empty vector were incubated with the proteasome inhibitor MG132 to block protein degradation. Cell lysates were then prepared and immunoprecipitationed with anti-Flag antibody were performed, and eluates were digested with trypsin and analyzed on a Q Exactive.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-25 | MSV000083160 | MassIVE
Sort   by:  
 Page size