RUF6 is a ncRNA gene family that is transcribed by RNA Polymerase III but actively regulates the Pol II-transcribed var virulence gene family. Understanding how RUF6 ncRNA connect to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in ...
During the dry season, in many African regions, persistent asymptomatic malaria cases have been linked to downregulated virulence gene expression encoded by the var gene family, resulting in low adhesion and increased circulation time of Plasmodium falciparum-infected erythrocytes 1. Several studies...
We adopted the technique of Chromatin Isolate by RNA Purification combined with high-throughput sequencing (ChIRP-seq) to gain mechanistic insight into the transcriptionally regulatory role of the trans-acting RUF6 ncRNA in detail
