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MetabolomicsSilage-derived flavonoids reprogram rumen microbiota to improve milk fat synthesis via rumen-mammary gland axis

Inoculation with homofermentative lactic acid bacteria (LAB) effectively enhances the silage quality of forages. Moreover, feeding such LAB-inoculated silage modulates rumen microbiota composition and metabolites, thereby improving ruminant production performance. Nevertheless, the specific mecha...

2026-01-11 | MTBLS13651 | MetaboLights
MetabolomicsRumen Fluid Metabolomics Analysis of Sheep Under Different Grazing Intensities with Supplementary Feeding

This dataset comprises untargeted LC-MS metabolomics profiles of rumen samples from 8 sheep under moderate grazing with supplementary feeding and 8 sheep under intensive grazing with supplementary feeding. Samples were analyzed using Thermo Fisher Scientific mass spectrometers in positive/negativ...

2025-07-20 | MTBLS12752 | MetaboLights
MetabolomicsIntegrative multi-omics analysis of rumen development in early-life yaks
This study investigates the developmental changes in the rumen of early-life yaks using integrative metagenomics, metabolomics, histology, fermentation profiling, and single-cell transcriptomics. Rumen fluid and epithelial tissue samples collected from yaks at 1, 6, and 12 months of age were analyze...
2026-04-21 | MTBLS14340 | MetaboLights
MetabolomicsProtozoal populations drive system-wide variation in the rumen microbiome

While rapid progress has been made to characterize the bacterial and archaeal populations of the rumen microbiome, insight into how they interact with keystone protozoal species remains elusive. Here, we reveal two distinct rumen community types (RCT-A and RCT-B) that are not strongly associated ...

2025-06-11 | MTBLS12283 | MetaboLights
MetabolomicsRumen unprotected glucose tends to promote intramuscular fat deposition via microbial volatile fatty acid-mediated metabolic reprogramming in cattle: A multi-omics perspective through the rumen-jejunum axis

Background: The regulatory effects of glucose absorption at different sites (rumen vs. small intestine) on lipid metabolism exhibit significant variation in beef nutrition. This study aimed to investigate the regulatory pathways of rumen-protected or unprotected glucose on lipid metabolism throug...

2026-03-25 | MTBLS14139 | MetaboLights
MetabolomicsEffect of Zanthoxylum bungeanum essential oil on rumen enzyme activity, microbiome, and metabolites in lambs

Antibiotics were once used in animal production to improve productivity and resistance to pathogenic microbiota. However, due to its negative effects, the search for a new class of substances that can replace its efficacy has become one of the urgent problems to be solved. Plant essential oils (E...

2022-09-13 | MTBLS3683 | MetaboLights
MetabolomicsSex-Driven Differences in the Rumen Microbe-Metabolite-Epithelial Transport Axis: An Integrated Multi-Omics Study of Rumen Function in Male and Female Zhongwei Goats
This study investigates sex-driven differences in rumen metabolism in male and female Zhongwei goats. Rumen fluid samples were analyzed using untargeted LC-MS metabolomics in both positive and negative ion modes. Metabolites were separated by ultra-high-performance liquid chromatography and detected...
2026-08-31 | MTBLS15319 | MetaboLights
MetabolomicsNon-targeted multimodal metabolomics and lipidomics data from ovine rumen fluid fractions

From an animal production and health perspective, our understanding of the metabolites in ruminant biofluids, particularly rumen fluid across different host species is poorly understood. Metabolomics is a powerful and sensitive approach for investigating low molecular weight metabolite profiles p...

2021-08-23 | MTBLS1717 | MetaboLights
MetabolomicsSilage-derived flavonoids reprogram rumen microbiota to improve milk fat synthesis via rumen-mammary gland axis

Inoculation with homofermentative lactic acid bacteria (LAB) effectively enhances the silage quality of forages. Moreover, feeding such LAB-inoculated silage modulates rumen microbiota composition and metabolites, thereby improving ruminant production performance. Nevertheless, the specific mecha...

2026-01-10 | MTBLS13648 | MetaboLights
MetabolomicsEffects of Lubabegron Fumarate on Ruminal Fermentation and Microbial Community in a Rumen Simulation System (MS (MTBLS))
Lubabegron fumarate (LBF), a novel β-adrenergic receptor modulator approved to mitigate these emissions, lacks a defined ruminal mode of action. This study aimed to characterise the effects of LBF on in vitro rumen fermentation, microbial ecology, and nitrogen metabolism.
2026-06-12 | MTBLS14756 | MetaboLights
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