EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in the pilot experiment [PXDxxx (PE083)]. Sample size: 12x12.
EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in a pilot experiment. Sample size: 12x12.
EndoC-bH1 cells proteomics was assessed in untreated baseline condition and after treatment with control siRNA at 24, 48 and 72 hours. Unpowered exploratory experiment. Sample size: triplicate per condition.
EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 24, 48 and 72 hours. Untreated baseline samples were also included. Unpowered exploratory experiment. Sample size: triplicate per condition.
Stable isotope-labeled standard (SIS) peptides are used as internal standards in targeted proteomics to provide robust protein quantification, which is required in clinical settings. However, SIS peptides are typically added post trypsin digestion and, as the digestion efficiency can vary significan...
