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TranscriptomicsGene Expression Profiles with Common Translocations in Acute Myeloid Leukemia (AML) using SAGE
SAGE was used to profile gene expression in leukemic myeloid progenitor cells of AML patients with t(8;21), t(15;17), t(9;11) and inv(16). Keywords: SAGE analysis of gene expression in leukemic myeloid progenitor cells isolated from bone marrow Bone marrow cells from 4 patients with de novo t(9;11),...
ORGANISM(S): Homo sapiens 
2005-11-30 | E-GEOD-3255 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)
Biomphalaria glabrata infection by the Schistosoma mansoni free-swimming miracidium and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and triggers a variety of physiological, biochemical and molecular changes. Here, we describe a genom...
ORGANISM(S): Schistosoma mansoni 
2009-03-09 | E-GEOD-9722 | biostudies-arrayexpress
TranscriptomicsRL-SAGE analysis of Zea mays leaf transcriptome
Using the RL-SAGE method (Gowda et al. 2004), a maize leaf longSAGE library (cv. inbred line B73) was constructed. Leaf tissues were harvested from 4-week old B73 plants for RNA isolation. The conditions in the growth chamber were 12 h light (500 µmol photons m-2 sec-1), 20oC at night, 26oC in the d...
ORGANISM(S): Zea mays 
2010-12-10 | E-GEOD-25994 | biostudies-arrayexpress
TranscriptomicsSAGE in Rhipicephalus (Boophilus) microplus following OP treatment of larvae from OP resistant/susceptible strains.
Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial Analysis of Gene Expression (SAGE) was used to analyze differential gene ...
ORGANISM(S): Rhipicephalus microplus 
2008-05-14 | E-GEOD-10109 | biostudies-arrayexpress
TranscriptomicsEvaluation of the chicken transcriptome by SAGE of B cell and the DT40 cell line
These libraries represent total RNA from bursal tissue of 20 day old CB-inbred chicks and the ALV induced bursal cell line DT40 Cre1 cells. Keywords = chicken transcriptome SAGE Bursal cell DT40
ORGANISM(S): Gallus gallus 
2004-10-06 | E-GEOD-1817 | biostudies-arrayexpress
TranscriptomicsSAGE of cotton fibers and ovules.
Transcriptomes fiber and ovules were compared by applying serial analysis of gene expression (SAGE). Keywords: Tissue Comparison We constructed three SAGE libraries and sequenced 57321, 64188, and 69104 tags from fiber, Xu-142 ovule (ovule) and fl mutant ovules (fl) respectively of Upland Cotton, G...
ORGANISM(S): Gossypium hirsutum 
2007-02-17 | E-GEOD-6103 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of human tissue samples using SAGE-Seq
We present a powerful application of ultra high-throughput sequencing, SAGE-Seq, for the accurate quantification of normal and neoplastic mammary epithelial cell transcriptomes. We develop data analysis pipelines that allow the mapping of sense and antisense strands of mitochondrial and RefSeq genes...
ORGANISM(S): Homo sapiens 
2010-10-05 | E-GEOD-24491 | biostudies-arrayexpress
TranscriptomicsGene regulated by ectopic expression of Sage in the entire Drosophila embryo
FoxA transcription factors play major roles in organ-specific gene expression. How FoxA proteins achieve specificity is unclear, given their broad expression patterns and requirements in multiple cell types. Here, we characterize Sage, a basic helix-loop-helix (bHLH) transcription factor expressed e...
ORGANISM(S): Drosophila melanogaster 
2013-06-06 | E-GEOD-40358 | biostudies-arrayexpress
TranscriptomicsDifferential SAGE analysis in Arabidopsis uncovers increased transcriptome complexity in response to low temperature
Five SAGE libraries were generated from A. thaliana leaf tissue collected at time points ranging from 30 minutes to one week of low temperature treatment (4°C). Over 240,000 high quality SAGE tags, corresponding to 16,629 annotated genes, provided a comprehensive survey of changes in the transcript...
ORGANISM(S): Arabidopsis thaliana 
2008-10-01 | E-GEOD-11461 | biostudies-arrayexpress
TranscriptomicsSAGE libraries derived from Bombyx ovaries
These two 3' Long SAGE libraries were constructed from stage 1-3 or stage 2-3 Bombyx egg chambers. Keywords: 3' Long SAGE libraries These libraries were constructed as described in Saha et al, 2002.
ORGANISM(S): Bombyx mori 
2006-10-31 | E-GEOD-5830 | biostudies-arrayexpress
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